Sagar Shweta, Pebam Monika, Sinha Rituparna, Rengan Aravind K, Panda Tarun K
Department of Chemistry, Indian Institute of Technology Hyderabad, Kandi 502284, Sangareddy, Telangana, India.
Department of Biomedical Engineering, Indian Institute of Technology Hyderabad, Kandi 502284, Sangareddy, Telangana, India.
ACS Omega. 2024 Mar 25;9(13):14860-14866. doi: 10.1021/acsomega.3c07898. eCollection 2024 Apr 2.
In the current research, dye-embedded polylactic acid (PLA) conjugate materials were synthesized using one-pot ring-opening polymerization (ROP), i.e., (dtHP) (2-[(2,4,6-trimethylphenyl) imino]-1(2)-acenaphthylenone-reduced-PLA) and (dmHP) (monoiminoacenaphtheneone-reduced-PLA), and then, nanoparticles (NPs) were engineered in the size range of 150 ± 30 nm. P(dtHP) NPs were employed in the treatment of melanoma, an aggressive type of skin cancer, which mandates the development of novel techniques to enhance healing outcomes and eliminate adverse effects related to existing treatments. In addition to exhibiting strong intracellular absorption in the spheroid model, the P(dtHP) NPs exhibited a strong cytotoxic effect on B16F10 cells, which resulted in oxidative stress from the generation of reactive oxygen species (ROS) and cell death. Additionally, a live/dead experiment using P(dtHP) NPs revealed a notable reduction in cell viability.
在当前研究中，采用一锅法开环聚合（ROP）合成了染料包埋的聚乳酸（PLA）共轭材料，即（dtHP）（2-[(2,4,6-三甲基苯基)亚氨基]-1(2)-苊烯酮还原-PLA）和（dmHP）（单亚氨基苊烯酮还原-PLA），然后制备了尺寸范围为150±30nm的纳米颗粒（NPs）。P（dtHP）NPs被用于治疗黑色素瘤，这是一种侵袭性皮肤癌，需要开发新技术来提高治疗效果并消除与现有治疗相关的不良反应。除了在球体模型中表现出强烈的细胞内吸收外，P（dtHP）NPs对B16F10细胞表现出强烈的细胞毒性作用，这导致活性氧（ROS）生成引起氧化应激和细胞死亡。此外，使用P（dtHP）NPs进行的活/死实验显示细胞活力显著降低。
