Lupulescu A
J Natl Cancer Inst. 1985 Jun;74(6):1335-46.
Long-term administration of prolactin (PRL) markedly enhanced the induction of epidermal squamous cell carcinomas by a chemical carcinogen, 3-methylcholanthrene [(MCA) CAS: 56-49-5], in Swiss male albino mice. DNA radioactivity and quantitative estimation of autoradiographs with the use of [3H]thymidine revealed a significant increase in DNA content (twofold) and in the percentage of labeled neoplastic nuclei in mice treated with PRL plus MCA (48.50%) as compared to that in mice treated with MCA alone (23.50%). Ultrastructural and cytologic studies revealed the predominance of a trabecular-hepatoid type of epidermal carcinoma with advanced nuclear irregularities, glycogen granules, mirror-image cells, and phagolysosomes in PRL-MCA-treated carcinomas as compared to characteristic squamous neoplastic cells with enlarged nuclei, tonofilaments, and keratin formation in epidermal carcinomas following treatment with MCA alone. Scanning electron microscopy revealed the predominance of rounded cells covered by numerous thick microvilli and blebs with an intense stromal reaction following PRL-MCA administration as compared to characteristic polyhedral cells covered by small microvilli following treatment with MCA alone. These findings demonstrate that PRL is an important hormone in epidermal neoplastic cell growth and differentiation.
在瑞士雄性白化小鼠中，长期给予催乳素（PRL）可显著增强化学致癌物3-甲基胆蒽[（MCA），CAS：56-49-5]诱导表皮鳞状细胞癌的能力。使用[3H]胸腺嘧啶核苷进行DNA放射性测定和放射自显影片的定量分析显示，与仅用MCA处理的小鼠相比，用PRL加MCA处理的小鼠的DNA含量显著增加（两倍），标记的肿瘤细胞核百分比也显著增加（48.50%对23.50%）。超微结构和细胞学研究表明，与仅用MCA处理后表皮癌中具有特征性的核增大、张力丝和角蛋白形成的鳞状肿瘤细胞相比，PRL-MCA处理的癌中以小梁-肝样型表皮癌为主，伴有晚期核不规则、糖原颗粒、镜像细胞和吞噬溶酶体。扫描电子显微镜显示，与仅用MCA处理后被小微绒毛覆盖的特征性多面体细胞相比，PRL-MCA给药后以被大量粗大微绒毛和泡覆盖的圆形细胞为主，伴有强烈的基质反应。这些发现表明，PRL是表皮肿瘤细胞生长和分化中的一种重要激素。
