Lupulescu A
J Natl Cancer Inst. 1983 Nov;71(5):1077-83.
Long-term administration of reserpine, an alkaloid, significantly retarded the induction of squamous cell carcinomas by a chemical carcinogen, 3-methylcholanthrene (MCA) in Swiss male albino mice. The incidence and development of carcinomas in mice simultaneously treated with reserpine and MCA was notably lowered as compared to those occurring in mice treated with MCA alone at any time interval. DNA synthesis and the percentage of labeled cells with [3H]thymidine was also significantly decreased in the nuclei of tumor cells following reserpine and MCA administration as compared to the findings for cells treated with MCA alone (18.50 and 39.00%, respectively). Electron microscopic and cytologic observations of tumors revealed the predominance of large areas of cytolysis and disintegration with an increase of lysosomes, myelin figures, and a decrease of mitochondria and polyribosome populations in tumor cells treated with reserpine and MCA, as compared to those results after treatment with MCA only. These findings demonstrate that reserpine significantly inhibited the carcinoma induction by a chemical carcinogen (MCA) in mice, possibly by inducing an advanced cytolysis and decreasing DNA synthesis in mouse tumor cells.
长期给予生物碱利血平，可显著延缓化学致癌物3-甲基胆蒽（MCA）在瑞士雄性白化小鼠中诱发鳞状细胞癌。与在任何时间间隔单独用MCA处理的小鼠相比，同时用利血平和MCA处理的小鼠中癌的发生率和发展明显降低。与单独用MCA处理的细胞相比（分别为18.50%和39.00%），给予利血平和MCA后肿瘤细胞核中的DNA合成以及用[3H]胸腺嘧啶标记的细胞百分比也显著降低。对肿瘤的电子显微镜和细胞学观察显示，与仅用MCA处理后的结果相比，在用利血平和MCA处理的肿瘤细胞中，大面积的细胞溶解和崩解占优势，溶酶体、髓鞘样结构增加，线粒体和多核糖体数量减少。这些发现表明，利血平可能通过诱导小鼠肿瘤细胞的晚期细胞溶解和减少DNA合成，显著抑制化学致癌物（MCA）在小鼠中诱发癌症。
