College of Medical Imaging Laboratory and Rehabilitation, Xiangnan University, Chenzhou, Hunan 423000, China.
Ruyuan Nanling Haoshanhaoshui Cordyceps Co., Ltd., Shaoguan, Guangdong 512000, China.
Anal Methods. 2024 Apr 25;16(16):2513-2521. doi: 10.1039/d4ay00170b.
Sweet tea is a functional herbal tea with anti-inflammatory, anti-diabetic, and other effects, in which phloridzin and trilobatin are two functional compounds. However, the current methods for their quantification are time-consuming, costly, and environmentally unfriendly. In this paper, we propose a rapid method that integrates online pressurized liquid extraction and high-performance liquid chromatography featuring a superficially porous column for fast separation. Moreover, we employ an equal absorption wavelength method to eliminate using multiple standard solutions and relative calibration factors. Our verification process corroborated the technique's selectivity, accuracy, precision, linearity, and detection limitations. Separately, our methodology demonstrated excellent analytical efficiency, cost-effectiveness, and environmental friendliness. Practical application using six distinct batches of sweet tea samples yielded results in congruence with the external standard method. The analytical rate of this technique is up to over 18 times faster than traditional methods, and organic solvent consumption has been reduced to less than 1.5 mL. Therefore, this method provides a valuable way to achieve quality control and green analysis of sweet tea and other herbal teas.
甜茶是一种具有抗炎、抗糖尿病等功效的功能性草本茶，其中根皮苷和三叶苷是两种具有功能的化合物。然而，目前对它们进行定量分析的方法既耗时、昂贵又对环境不友好。在本文中，我们提出了一种快速方法，该方法集成了在线加压液体萃取和高效液相色谱法，采用表面多孔柱进行快速分离。此外，我们采用等吸收波长法消除了使用多个标准溶液和相对校准因子的需要。我们的验证过程证实了该技术的选择性、准确性、精密度、线性和检测极限。此外，我们的方法还表现出出色的分析效率、成本效益和环境友好性。使用六个不同批次的甜茶样品进行实际应用，结果与外标法一致。该技术的分析速度比传统方法快 18 倍以上，有机溶剂的消耗减少到 1.5 毫升以下。因此，该方法为甜茶和其他草本茶的质量控制和绿色分析提供了一种有价值的方法。
