Department of Pharmacy, School of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Hunan Collaborative Innovation Center for Effective Utilizing of Wood & Bamboo Resources, Central South University of Forestry and Technology, Changsha, China.
J Food Biochem. 2021 Mar;45(3):e13183. doi: 10.1111/jfbc.13183. Epub 2020 Mar 16.
This paper aims to investigate the constituent phytochemicals and biological activities of six Lithocarpus litseifolius (Hance) Chun (L. litseifolius) cultivars. Processed by UPLC-MS/MS and untargeted metabolomics analysis software (MS-DIAL and MS-FINDER), components of different L. litseifolius cultivars were analyzed qualitatively and quantitatively. By conducting a comprehensive analysis of spectra, MS/MS fragmentation, and retention time, 40 compounds (including 6 phenolic glycosides and 34 flavonoids) were identified rapidly and accurately. Moreover, the total phenolic content (TPC), total flavonoid content (TFC), antioxidant, antimicrobial effects, and pheochromocytoma (PC12) cell viability of different L. litseifolius varieties were evaluated. Purple small leaf (PSL) was found to be the most superior variety with the highest antioxidant activities (8.27, 10.09, and 10.80 mg TE/g for DPPH, ABTS, and FRAP assays, respectively), broad-spectrum antimicrobial activity, and lowest cell cytotoxicity. The results showed that these bioactivities were closely related to phytochemicals among the six cultivars. PRACTICAL APPLICATIONS: L. litseifolius is well known as an important folk drink in the Southern China folklore for its specific efficacy in preventing diabetes. However, there is still a paucity of information on L. litseifolius, as other active components and bioactivities of it remain to be investigated. The wide range of flavonoids discovered in L. litseifolius provides abundant natural health-promoting agents for further applications in medicine and functional food.
本研究旨在探究 6 个锥栗（Lithocarpus litseifolius（Hance）Chun，L. litseifolius）品种的植物化学成分和生物活性。通过 UPLC-MS/MS 和非靶向代谢组学分析软件（MS-DIAL 和 MS-FINDER）对不同锥栗品种的成分进行定性和定量分析。通过综合分析光谱、MS/MS 碎片和保留时间，快速准确地鉴定出 40 种化合物（包括 6 种酚糖苷和 34 种类黄酮）。此外，还评估了不同锥栗品种的总酚含量（TPC）、总黄酮含量（TFC）、抗氧化、抗菌作用和嗜铬细胞瘤（PC12）细胞活力。结果表明，紫叶小果（PSL）是表现最优异的品种，具有最高的抗氧化活性（DPPH、ABTS 和 FRAP 测定法分别为 8.27、10.09 和 10.80 mg TE/g）、广谱抗菌活性和最低的细胞毒性。研究结果表明，这些生物活性与 6 个品种中的植物化学成分密切相关。实际应用：锥栗作为中国南方民间防治糖尿病的一种重要民间饮品，具有独特的功效而广为人知。然而，关于锥栗的信息仍然很少，因为其其他活性成分和生物活性仍有待研究。在锥栗中发现的广泛存在的类黄酮为进一步应用于医学和功能性食品提供了丰富的天然健康促进剂。
