Longhua Hospital, Shanghai University of Traditional Chinese Medicine; Key Laboratory of theory and therapy of muscles and bones, Ministry of Education.
Longhua Hospital, Shanghai University of Traditional Chinese Medicine; Key Laboratory of theory and therapy of muscles and bones, Ministry of Education;
J Vis Exp. 2024 Mar 22(205). doi: 10.3791/66491.
Osteosarcoma is the most common primary malignant bone tumor in children and adolescents. Despite the development of new treatment plans in recent years, the prognosis for osteosarcoma patients has not significantly improved. Therefore, it is crucial to establish a robust preclinical model with high fidelity. The patient-derived xenograft (PDX) model faithfully preserves the genetic, epigenetic, and heterogeneous characteristics of human malignancies for each patient. Consequently, PDX models are considered authentic in vivo models for studying various cancers in transformation studies. This article presents a comprehensive protocol for creating and maintaining a PDX mouse model that accurately mirrors the morphological features of human osteosarcoma. This involves the immediate transplantation of freshly resected human osteosarcoma tissue into immunocompromised mice, followed by successive passaging. The described model serves as a platform for studying the growth, drug resistance, relapse, and metastasis of osteosarcoma. Additionally, it aids in screening the target therapeutics and establishing personalized treatment schemes.
骨肉瘤是儿童和青少年中最常见的原发性恶性骨肿瘤。尽管近年来新的治疗方案不断发展，但骨肉瘤患者的预后并没有显著改善。因此，建立一个具有高度保真度的强大临床前模型至关重要。患者来源的异种移植（PDX）模型忠实地保留了每个患者恶性肿瘤的遗传、表观遗传和异质性特征。因此，PDX 模型被认为是研究各种癌症转化研究的真实体内模型。本文介绍了一种创建和维持 PDX 小鼠模型的综合方案，该模型准确地模拟了人类骨肉瘤的形态特征。这涉及将新鲜切除的人类骨肉瘤组织立即移植到免疫功能低下的小鼠中，然后进行连续传代。所描述的模型可用于研究骨肉瘤的生长、耐药性、复发和转移。此外，它还有助于筛选靶向治疗药物并制定个性化治疗方案。
