类胡萝卜素和生育酚在肉质果实中的分析。
Carotenoids and Tocopherol Profiling in Fleshy Fruits.
机构信息
Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.
出版信息
Methods Mol Biol. 2024;2798:141-151. doi: 10.1007/978-1-0716-3826-2_9.
PMID:38587740
Abstract
Carotenoids and tocopherols are among the most powerful lipophilic antioxidants accumulated in fruit and vegetable crops. This chapter describes a method for the separation and quantification of carotenoids/chlorophylls and tocopherols based on microextraction followed by reverse- and normal-phase HPLC, respectively. Using this method, high-throughput, accurate analysis of these compounds can be performed in leaf and fruit samples.
摘要
类胡萝卜素和生育酚是在水果和蔬菜作物中积累的最有效的脂溶性抗氧化剂之一。本章描述了一种基于微萃取，分别采用反相和正相 HPLC 分离和定量类胡萝卜素/叶绿素和生育酚的方法。使用这种方法，可以在叶片和果实样品中进行高通量、准确地分析这些化合物。
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