Department of Plant Medicine, National Chiayi University, Chiayi City 600355, Taiwan.
Plant Dis. 2024 Sep;108(9):2688-2700. doi: 10.1094/PDIS-12-22-2937-RE. Epub 2024 Aug 12.
The tomato yellow leaf curl disease (TYLCD) caused by whitefly ()-transmitted begomoviruses () has constrained tomato production in Taiwan since 1981. Lisianthus enation leaf curl virus (LELCV), tomato leaf curl Taiwan virus (ToLCTV), and tomato yellow leaf curl Thailand virus (TYLCTHV) were the major viruses associated with TYLCD. In 2019 to 2020, we investigated TYLCD throughout Taiwan, with a 10 to 100% incidence on tomato fields. Begomovirus sequences were detected in 321 out of 506 collected samples by PCR with primers PAL1v1978B and PAR1c715H. In 2015 to 2016, 59 out of 99 samples collected in Hualien-Taitung areas were also found to have begomovirus sequences. Based on the analysis of 68 viral genomic sequences, six begomoviruses were identified, including LELCV, ToLCTV, TYLCTHV, tomato leaf curl Hsinchu virus, and two new begomoviruses, tentatively named tomato leaf curl Chiayi virus (ToLCCYV) and tomato leaf curl Nantou virus (ToLCNTV). Various isolates of LELCV and TYLCTHV were grouped into four and two strains, respectively. Recombinants were detected in LELCV-A, -C, and -D, ToLCCYV, ToLCNTV, and TYLCTHV-F. Based on virus-specific detection, the majority of TYLCD-associated viruses were mixed-infected by TYLCTHV-B with TYLCTHV-F, LELCV-A, -B, or -D, and/or ToLCTV. Meanwhile, viral DNA-B was mostly associated with TYLCTHV, and all identified DNA-Bs were highly homologous with previous TYLCTHV DNA-B. The pathogenicity of selected begomoviruses was confirmed through agroinfection and whitefly transmission. All tomato plants carrying / and resistant genes were infected by all LELCV strains and ToLCCYV, although they appeared symptomless, suggesting these viruses could be managed through the use of the resistance pyramid.
自 1981 年以来，由烟粉虱传播的番茄黄曲叶病（TYLCD）一直制约着台湾的番茄生产。百合潜隐病毒（LELCV）、番茄曲叶台湾病毒（ToLCTV）和番茄黄曲叶泰国病毒（TYLCTHV）是与 TYLCD 相关的主要病毒。2019 年至 2020 年，我们对台湾各地的 TYLCD 进行了调查，番茄田的发病率为 10%至 100%。通过引物 PAL1v1978B 和 PAR1c715H 的 PCR 检测，从 506 个采集样本中检测到 321 个分离物呈阳性。2015 年至 2016 年，在花莲-台东地区采集的 99 个样本中也发现了烟粉虱携带的病毒序列。基于对 68 个病毒基因组序列的分析，鉴定出 6 种曲叶病毒，包括 LELCV、ToLCTV、TYLCTHV、新竹番茄曲叶病毒和两种新的曲叶病毒，暂定名为嘉义番茄曲叶病毒（ToLCCYV）和南投番茄曲叶病毒（ToLCNTV）。不同分离株的 LELCV 和 TYLCTHV 分别分为 4 个和 2 个株系。在 LELCV-A、-C 和 -D、ToLCCYV、ToLCNTV 和 TYLCTHV-F 中检测到重组体。基于病毒特异性检测，与 TYLCD 相关的大多数病毒是由 TYLCTHV-B 与 TYLCTHV-F、LELCV-A、-B 或 -D 和/或 ToLCTV 混合感染的。同时，病毒 DNA-B 主要与 TYLCTHV 相关，所有鉴定的 DNA-B 与之前的 TYLCTHV DNA-B 高度同源。通过农杆菌感染和粉虱传播证实了所选曲叶病毒的致病性。所有携带 / 和 抗性基因的番茄植株均被所有 LELCV 株系和 ToLCCYV 感染，尽管它们没有出现症状，但这表明可以通过使用抗性金字塔来管理这些病毒。
