Chen Chen, Yurtsever Ayhan, Li Peiying, Sun Linhao
Earth-Life Science Institute, Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8550, Japan.
WPI Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
ACS Appl Mater Interfaces. 2024 Apr 17;16(15):19699-19710. doi: 10.1021/acsami.3c18851. Epub 2024 Apr 8.
Self-assembly of peptides on layered nanomaterials such as graphite and MoS in the formation of long-range ordered two-dimensional nanocrystal patterns leading to its potential applications for biosensing and bioelectronics has attracted significant interest in nanoscience and nanotechnology. However, controlling the self-assembly of peptides on nanomaterials is still challenging due to the unclear role of nanomaterials in steering self-assembly. Here, we used the AFM technique to capture different changes of peptide coverage as well as lengthening and widening rates depending on peptide concentrations, show the distinct boundary dynamics of two stabilized peptide domains, and resolve the molecular resolution structural differences and specific orientation of peptide on both nanomaterials. Moreover, results showed that the nanomaterial layers tuned the opposite changes of nanowire heights and densities and displayed the different water-resistance stabilities on both nanomaterials. This work provides a basis for understanding nanomaterials steering peptide self-assembly and using hybrid bionanomaterials as a scaffold, enabling for potential biosensing and bioelectronics applications.
肽在层状纳米材料（如石墨和二硫化钼）上自组装形成长程有序的二维纳米晶体图案，这使其在生物传感和生物电子学方面具有潜在应用，从而在纳米科学和纳米技术领域引起了极大兴趣。然而，由于纳米材料在引导自组装过程中的作用尚不清楚，控制肽在纳米材料上的自组装仍然具有挑战性。在这里，我们使用原子力显微镜技术来捕捉肽覆盖率的不同变化以及取决于肽浓度的伸长和加宽速率，展示两个稳定肽结构域的明显边界动态，并解析肽在两种纳米材料上的分子分辨率结构差异和特定取向。此外，结果表明纳米材料层调节了纳米线高度和密度的相反变化，并在两种纳米材料上表现出不同的耐水稳定性。这项工作为理解纳米材料引导肽自组装以及使用混合生物纳米材料作为支架提供了基础，从而实现潜在的生物传感和生物电子学应用。
