Harigaya K, Handa H
Proc Natl Acad Sci U S A. 1985 May;82(10):3477-80. doi: 10.1073/pnas.82.10.3477.
Five clonal human bone marrow stromal cell lines were isolated from the adherent cell populations in long-term liquid cultures after transfection with the recombinant plasmid pSV3gpt. All the cell-line feeder layers and their conditioned media stimulated the proliferation of committed granulomonocytic stem cells (CFUc) from human bone marrow. The size and number of early erythroid stem cell (BFUe)-derived colonies were significantly increased when in the presence of 10% conditioned medium from the cell lines. Furthermore, a substantial number of mixed colonies with erythroid components were observed in the cultures in the presence of erythropoietin and conditioned medium. These findings suggest that the human bone marrow stromal cell lines obtained after transfection with pSV3gpt may be extremely useful in identifying the hematopoietic factors derived from the hematopoietic microenvironment and in analyzing their mechanism of action.
用重组质粒pSV3gpt转染后，从长期液体培养中的贴壁细胞群体中分离出5个人类骨髓基质细胞克隆系。所有细胞系饲养层及其条件培养基均刺激人骨髓中定向粒单核干细胞（CFUc）的增殖。当存在来自细胞系的10%条件培养基时，早期红系干细胞（BFUe）衍生集落的大小和数量显著增加。此外，在存在促红细胞生成素和条件培养基的培养物中观察到大量含有红系成分的混合集落。这些发现表明，用pSV3gpt转染后获得的人类骨髓基质细胞克隆系可能在鉴定源自造血微环境的造血因子及其作用机制分析方面极其有用。
