Kodama H A, Amagai Y, Koyama H, Kasai S
J Cell Physiol. 1982 Jul;112(1):83-8. doi: 10.1002/jcp.1041120113.
We established a clonal preadispose cell line from newborn mouse calvaria. Cells of this cell line, designated MC3T3-G2/PA6, had the capacity to convert to adipose cells, to accumulate triglycerides in their cytoplasm, and to mature to differentiated fat cells in a resting state. This adipose conversion was markedly accelerated by addition of dexamethasone, which was the most potent inducer among the steroid hormones tested. The presence of dexamethasone was needed during the steroid hormones tested. The presence of dexamethasone was needed during logarithmic growth phase for maximal conversion. The frequency of adipose conversion was dependent on exposure time to the hormone, but cells already committed to differentiation continued to accumulate lipid and developed into mature adipose cell even in its absence. This indicates that the hormone accelerates the initiation of the adipose conversion, but is not required for the ongoing conversion process. In fact, it was rather inhibitory for the process of fat accumulation. Insulin alone slightly inhibited the adipose conversion, but its combination with dexamethasone neutralized the above inhibitory effect of dexamethasone. The responsiveness of this cell line is consistent with that observed for mouse bone marrow preadipocytes in primary culture but differs from that for preadipose cell lines derived from extramedullary tissues. These results strongly suggest that the MC3T3-G2/PA6 cell line was derived from bone marrow.
我们从新生小鼠颅骨建立了一种克隆性前体脂肪细胞系。该细胞系的细胞，命名为MC3T3-G2/PA6，具有转化为脂肪细胞、在细胞质中积累甘油三酯以及在静止状态下成熟为分化脂肪细胞的能力。添加地塞米松可显著加速这种脂肪转化，地塞米松是所测试的类固醇激素中最有效的诱导剂。在所测试的类固醇激素作用期间需要有地塞米松存在。在对数生长期需要有地塞米松存在以实现最大程度的转化。脂肪转化的频率取决于激素暴露时间，但已决定分化的细胞即使在没有激素的情况下也会继续积累脂质并发育成成熟脂肪细胞。这表明该激素加速脂肪转化的起始，但在持续的转化过程中并非必需。实际上，它对脂肪积累过程有一定抑制作用。单独使用胰岛素对脂肪转化有轻微抑制作用，但其与地塞米松联合使用可抵消地塞米松的上述抑制作用。该细胞系的反应性与原代培养的小鼠骨髓前脂肪细胞所观察到的一致，但与源自髓外组织的前脂肪细胞系不同。这些结果强烈表明MC3T3-G2/PA6细胞系源自骨髓。
