Shipley G D, Tucker R F, Moses H L
Proc Natl Acad Sci U S A. 1985 Jun;82(12):4147-51. doi: 10.1073/pnas.82.12.4147.
Type beta transforming growth factor/growth inhibitor (TGF-beta/GI) is demonstrated to be a potent stimulator of DNA synthesis in AKR-2B mouse embryo cells with a prolonged (greater than 24 hr) prereplicative phase when compared with other growth factors (epidermal growth factor, platelet-derived growth factor, or fibroblast growth factor) that induce DNA synthesis 12-14 hr after stimulation. In addition, TGF-beta/GI inhibits the early peak of DNA synthesis produced by EGF and insulin before the later stimulatory effects of TGF-beta/GI become manifest. TGF-beta/GI induces a marked morphologic transformation in these cells prior to their entry into S phase. Like the other growth factors, TGF-beta/GI stimulates an early increase in the rate of protein synthesis in AKR-2B cells and its stimulatory effect on DNA synthesis is enhanced by insulin. The data show that this molecule is a growth factor for certain mesenchymal cells in monolayer culture but only after a prereplicative phase that is significantly longer than that of other growth factors.
β型转化生长因子/生长抑制剂(TGF-β/GI)被证明是AKR-2B小鼠胚胎细胞中DNA合成的有效刺激因子,与其他生长因子(表皮生长因子、血小板衍生生长因子或成纤维细胞生长因子)相比,其复制前期延长(大于24小时),而其他生长因子在刺激后12 - 14小时诱导DNA合成。此外,在TGF-β/GI后期的刺激作用显现之前,它会抑制由表皮生长因子(EGF)和胰岛素产生的DNA合成早期峰值。TGF-β/GI在这些细胞进入S期之前诱导明显的形态转化。与其他生长因子一样,TGF-β/GI刺激AKR-2B细胞中蛋白质合成速率早期增加,并且胰岛素会增强其对DNA合成的刺激作用。数据表明,该分子是单层培养中某些间充质细胞的生长因子,但仅在经历比其他生长因子长得多的复制前期之后。