肾上皮细胞生长抑制剂的纯化
Purification of kidney epithelial cell growth inhibitors.
作者信息
Holley R W, Böhlen P, Fava R, Baldwin J H, Kleeman G, Armour R
出版信息
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5989-92. doi: 10.1073/pnas.77.10.5989.
Abstract
Two high molecular weight growth inhibitors have been isolated from the culture medium of BSC-1 cells, epithelial cells of African green monkey kidney. The purified kidney epithelial cell growth inhibitors, at ng/ml concentrations, reversibly arrest the growth of BSC-1 cells in the G1 phase of the cell cycle. Their action is selective; they are most active on BSC-1 cells, are less active as inhibitors of the growth of rat lung and human breast epithelial cells, and do not inhibit the growth of 3T3 mouse embryo fibroblasts ad human skin fibroblasts in culture. Their growth inhibitory action on BSC-1 cell cultures is counteracted by epidermal growth factor or calf serum.
摘要
从非洲绿猴肾上皮细胞系BSC-1细胞的培养基中分离出了两种高分子量生长抑制剂。纯化后的肾上皮细胞生长抑制剂在纳克/毫升浓度下，可使BSC-1细胞在细胞周期的G1期生长可逆性停滞。它们的作用具有选择性；对BSC-1细胞活性最强，作为大鼠肺和人乳腺上皮细胞生长的抑制剂活性较弱，并且在培养中不抑制3T3小鼠胚胎成纤维细胞和人皮肤成纤维细胞的生长。表皮生长因子或小牛血清可抵消它们对BSC-1细胞培养物的生长抑制作用。
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