Ssedyabane Frank, Ngonzi Joseph, Tusubira Deusdedit, Nambi Najjuma Josephine, Kajabwangu Rogers, Okeny Christopher, Nuwashaba Doreen, Namuli Alexcer, Niyonzima Nixon
Department of Medical Laboratory Science, Faculty of Medicine, Mbarara University of Science of Science and Technology, P.O. Box 1410, Mbarara, Uganda.
Department of Obstetrics and Gynecology, Mbarara University of Science of Science and Technology, P.O. Box 1410, Mbarara, Uganda.
Gynecol Oncol Rep. 2024 Mar 31;53:101388. doi: 10.1016/j.gore.2024.101388. eCollection 2024 Jun.
Tissue expression of P16ink4A is correlated with cervical lesions. In this study we determined the association between serum P16ink4A concentrations and cervical lesions among women attending the cervical cancer clinic at Mbarara Regional Hospital (MRRH) South Western Uganda.
We recruited 90 cervical intraepithelial neoplasia (CIN) cases, 90 cervical cancer (CC) cases before treatment and 90 controls. Clinical and demographic data were recorded. Serum P16ink4A concentrations were measured by quantitative Elisa. Cases were confirmed with cytology and/or histology. Descriptive statistics and logistic regression were done with STATA 17 and P-values of <0.05 were considered statistically significant.
The mean serum P16ink4A concentration among CIN cases, CC cases and controls was 1.11(+/-0.66) ng/ml, 1.45(+/-1.11) ng/ml and 1.13(+/-0.61) ng/ml respectively (p = 0.008). 50 % of CIN cases and controls as well as 60 % of CC cases had P16ink4A concentration above 0.946 ng/ml. There were increased odds of CIN for serum P16ink4A though statistically insignificant (AOR: 1.11, p-value: 0.70). There was also a statistically significant reduction in odds of CC for serum P16ink4A (AOR: 0.55, p-value: 0.01).
Serum P16ink4A may likely be associated with cervical lesions especially CC in our study population and this may aid detection of such lesions. Diagnostic utility studies for circulating P16ink4A in detection of cervical cancer are recommended.
P16ink4A的组织表达与宫颈病变相关。在本研究中，我们确定了乌干达西南部姆巴拉拉地区医院（MRRH）宫颈癌门诊女性血清P16ink4A浓度与宫颈病变之间的关联。
我们招募了90例宫颈上皮内瘤变（CIN）病例、90例治疗前宫颈癌（CC）病例和90例对照。记录临床和人口统计学数据。通过定量酶联免疫吸附测定法测量血清P16ink4A浓度。病例通过细胞学和/或组织学确诊。使用STATA 17进行描述性统计和逻辑回归分析，P值<0.05被认为具有统计学意义。
CIN病例、CC病例和对照的血清P16ink4A平均浓度分别为1.11（±0.66）ng/ml、1.45（±1.11）ng/ml和1.13（±0.61）ng/ml（p = 0.008）。50%的CIN病例和对照以及60%的CC病例的P16ink4A浓度高于0.946 ng/ml。血清P16ink4A浓度升高时CIN的发病几率增加，尽管无统计学意义（调整后比值比：1.11，P值：0.70）。血清P16ink4A浓度升高时CC的发病几率也有统计学意义的降低（调整后比值比：0.55，P值：0.01）。
在我们的研究人群中，血清P16ink4A可能与宫颈病变尤其是CC相关，这可能有助于此类病变的检测。建议开展关于循环P16ink4A在宫颈癌检测中的诊断效用研究。
