一锅等温扩增允许 CRISPR/Cas12a 的循环激活，用于检测末端脱氧核苷酸转移酶活性。
One-pot isothermal amplification permits recycled activation of CRISPR/Cas12a for sensing terminal deoxynucleotidyl transferase activity.
机构信息
College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang 314001, P. R. China.
College of Materials and Chemical Engineering, Minjiang University, Fuzhou 350108, Fujian, China.
出版信息
Chem Commun (Camb). 2024 Apr 25;60(35):4683-4686. doi: 10.1039/d4cc00825a.
This study introduces a one-pot isothermal amplification assay for ultrasensitive analysis of terminal deoxynucleotidyl transferase (TdT) activity. The system realizes recycled activation of CRISPR/Cas12a, enabling exceptional signal amplification. This approach maximizes the simplicity of the detection method, offering a promising avenue for molecular disease diagnosis.
本研究介绍了一种用于端脱氧核苷酸转移酶（TdT）活性超灵敏分析的一锅同温扩增测定法。该系统实现了 CRISPR/Cas12a 的循环激活，从而实现了卓越的信号放大。这种方法最大限度地简化了检测方法，为分子疾病诊断提供了有前途的途径。
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