Xie Xi, Lin Miaoyan, Xiao Gengsheng, Wang Qin, Li Zhiyong
Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Department of Biology, Institute of Plant and Food Science, Southern University of Science and Technology, Shenzhen 518055, China.
Plants (Basel). 2024 Mar 8;13(6):774. doi: 10.3390/plants13060774.
/ (ABA response element binding) proteins in plants are essential for stress responses, while our understanding of / from orchid species, important traditional medicinal and ornamental plants, is limited. Here, twelve / genes were identified within three orchids' complete genomes and classified into three groups through phylogenetic analysis, which was further supported with a combined analysis of their conserved motifs and gene structures. The -element analysis revealed that hormone response elements as well as light and stress response elements were widely rich in the s. A prediction analysis of the orchid /-mediated regulatory network was further constructed through -regulatory element (CRE) analysis of their promoter regions. And it revealed that several dominant transcriptional factor (TF) gene families were abundant as potential regulators of these orchid s. Expression profile analysis using public transcriptomic data suggested that most genes have distinct tissue-specific expression patterns in orchid plants. Additionally, DcaABI5 as a homolog of ABA INSENSITIVE 5 (ABI5) from was selected for further analysis. The results showed that transgenic overexpressing could rescue the ABA-insensitive phenotype in the mutant . Collectively, these findings will provide valuable information on genes in orchids.
植物中的(脱落酸应答元件结合)蛋白对于应激反应至关重要,而我们对作为重要传统药用和观赏植物的兰花物种中的此类蛋白了解有限。在此,在三种兰花的完整基因组中鉴定出了12个此类基因,并通过系统发育分析将其分为三组,对其保守基序和基因结构的综合分析进一步支持了这一分类。元件分析表明,激素应答元件以及光和应激应答元件在这些基因中广泛丰富。通过对其启动子区域的顺式作用元件(CRE)分析,进一步构建了兰花此类蛋白介导的调控网络的预测分析。结果表明,几个主要的转录因子(TF)基因家族作为这些兰花此类蛋白的潜在调节因子大量存在。使用公开的转录组数据进行的表达谱分析表明,大多数此类基因在兰花植物中具有明显的组织特异性表达模式。此外,选择来自石斛的DcaABI5作为脱落酸不敏感5(ABI5)的同源物进行进一步分析。结果表明,过表达DcaABI5的转基因石斛可以挽救突变体中的脱落酸不敏感表型。总体而言,这些发现将为兰花中的此类基因提供有价值的信息。