He Zhilong, Zhang Ying, Xun Chengfeng, Yang Dayu, Zhang Zhen, Ma Yushen, Wei Xin, Wan Zhentao, Wang Xiangnan, Zhang Yufeng, Chen Yongzhong, Wang Rui
Research Institute of Oil Tea Camellia, Hunan Academy of Forestry, Changsha 410004, China.
Yuelushan Laboratory, Changsha 410004, China.
Plants (Basel). 2025 Aug 18;14(16):2568. doi: 10.3390/plants14162568.
As a key economic tree in southern China, faces severe yield losses under drought. Grafting onto drought-tolerant rootstocks offers a potential mitigation strategy. To elucidate the impact of rootstocks on the drought resistance of the superior Abel. cultivar "Xianglin 210", grafted seedlings with five scion-rootstock combinations, were subjected to gradient drought stress. Key physiological and biochemical indices related to photosynthesis, antioxidant enzymes, and osmotic adjustment were measured. Drought resistance was comprehensively evaluated using membership function analysis, and the expression of stress-responsive genes was quantified via quantitative real-time PCR (qRT-PCR). The results demonstrated that under drought stress, (1) stomatal conductance (Gs) decreased by 31.2-48.7%, while instantaneous water use efficiency (WUE) increased by 18.5-35.4%; (2) proline (Pro) and soluble sugars (SS) accumulated significantly, with increases of 2.3-4.1-fold and 1.8-3.2-fold, respectively; (3) activities of antioxidant enzymes were enhanced by 56-127%, mitigating oxidative damage; (4) membership function analysis ranked drought resistance as follows: Xianglin 27 (0.812) > Guangxi Superior Germplasm (0.698) > C. yuhsienensis (0.654) > Hunan Superior Germplasm (0.591) > Xianglin 1 (0.523); (5) qRT-PCR revealed significant upregulation of ABA signaling pathway genes (, , , and ) and transcription factors ( and ), indicating activation of drought-responsive regulatory networks. These findings provide a theoretical foundation for selecting drought-tolerant rootstocks and optimizing cultivation practices in , and provide practical criteria for selecting drought-tolerant rootstocks, facilitating sustainable cultivation in water-limited regions.
作为中国南方的一种关键经济树种，[树种名称]在干旱条件下产量损失严重。嫁接到耐旱砧木上是一种潜在的缓解策略。为了阐明砧木对优良[树种名称]品种“湘林210”抗旱性的影响，对具有五种接穗 - 砧木组合的嫁接苗进行了梯度干旱胁迫处理。测量了与光合作用、抗氧化酶和渗透调节相关的关键生理生化指标。使用隶属函数分析对抗旱性进行了综合评价，并通过定量实时PCR（qRT-PCR）对胁迫响应基因的表达进行了定量分析。结果表明，在干旱胁迫下，（1）气孔导度（Gs）下降了31.2 - 48.7%，而瞬时水分利用效率（WUE）提高了18.5 - 35.4%；（2）脯氨酸（Pro）和可溶性糖（SS）显著积累，分别增加了2.3 - 4.1倍和1.8 - 3.2倍；（3）抗氧化酶活性提高了56 - 127%，减轻了氧化损伤；（4）隶属函数分析得出的抗旱性排名如下：湘林27（0.812）>广西优良种质（0.698）>玉屏油茶（0.654）>湖南优良种质（0.591）>湘林1（0.523）；（5）qRT-PCR显示脱落酸信号通路基因（[具体基因名称1]、[具体基因名称2]、[具体基因名称3]和[具体基因名称4]）和转录因子（[具体转录因子名称1]和[具体转录因子名称2]）显著上调，表明干旱响应调控网络被激活。这些发现为[树种名称]选择耐旱砧木和优化栽培实践提供了理论基础，并为选择耐旱砧木提供了实用标准，有助于在水资源有限地区实现[树种名称]的可持续栽培。
