Laboratory of Neuroinformatics, Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, Poland.
Neurobiology of Emotions Laboratory, Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, Poland.
PLoS One. 2024 Apr 9;19(4):e0301713. doi: 10.1371/journal.pone.0301713. eCollection 2024.
Local Field Potential (LFP), despite its name, often reflects remote activity. Depending on the orientation and synchrony of their sources, both oscillations and more complex waves may passively spread in brain tissue over long distances and be falsely interpreted as local activity at such distant recording sites. Here we show that the whisker-evoked potentials in the thalamic nuclei are of local origin up to around 6 ms post stimulus, but the later (7-15 ms) wave is overshadowed by a negative component reaching from cortex. This component can be analytically removed and local thalamic LFP can be recovered reliably using Current Source Density analysis. We used model-based kernel CSD (kCSD) method which allowed us to study the contribution of local and distant currents to LFP from rat thalamic nuclei and barrel cortex recorded with multiple, non-linear and non-regular multichannel probes. Importantly, we verified that concurrent recordings from the cortex are not essential for reliable thalamic CSD estimation. The proposed framework can be used to analyze LFP from other brain areas and has consequences for general LFP interpretation and analysis.
局部场电位（LFP），尽管其名称如此，却常常反映的是远程活动。根据其来源的方向和同步性，无论是振荡还是更复杂的波都可能在脑组织中被动地远距离传播，并被错误地解释为在这些遥远的记录部位的局部活动。在这里，我们表明，在刺激后约 6 毫秒，触须诱发的丘脑核中的电位是局部起源的，但随后（7-15 毫秒）的波被从皮层延伸而来的负成分所掩盖。可以分析去除这个成分，并使用电流源密度分析（CSD）可靠地恢复局部丘脑 LFP。我们使用基于模型的核电流源密度分析（kCSD）方法，该方法允许我们研究来自大鼠丘脑核和桶状皮层的 LFP 的局部和远程电流的贡献，这些记录是使用多个非线性和不规则的多通道探头进行的。重要的是，我们验证了来自皮层的并发记录对于可靠的丘脑 CSD 估计并不是必需的。所提出的框架可用于分析来自其他脑区的 LFP，并对一般的 LFP 解释和分析产生影响。
