碱性成纤维细胞生长因子/壳聚糖衍生物/胶原复合温敏水凝胶促进大鼠牙周组织再生。

Basic fibroblast growth factor/chitosan derivatives/collagen composite thermosensitive hydrogel promotes perio-dontal tissue regeneration in rats.

机构信息

Dept. of Endodontics, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou 510182, China.

Dept. of Stomatology, Liaocheng People's Hospital, Liaocheng 252020, China.

出版信息

Hua Xi Kou Qiang Yi Xue Za Zhi. 2023 Feb 1;41(1):21-28. doi: 10.7518/hxkq.2023.01.003.

DOI:10.7518/hxkq.2023.01.003

PMID:38596937

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9988450/

Abstract

OBJECTIVES

To investigate the feasibility of different thermosensitive composite hydrogels from chitosan derivatives as scaffold materials for periodontal tissue engineering.

METHODS

Three chitosan derivatives with different biological characteristics were prepared, namely, sulfonated chitosan (SCS), phosphorylated chitosan (PCS), and phosphorylated sulfonated chitosan (PSCS). Three thermosensitive composite hydrogels were constructed using basic fibroblast growth factor (bFGF), the chitosan derivatives, and collagen. Twenty male Wistar rats were randomly divided into control group, blank group, bFGF/SCS/collagen composite thermosensitive hydrogel group, bFGF/PCS/collagen compo-site thermosensitive hydrogel group, and bFGF/PSCS/collagen composite thermosensitive hydrogel group. Then, three-wall intrabony defects were established. The defects were treated with the different kinds of thermosensitive composite hydrogels. After 6 weeks of surgery, the animals were killed, and specimens were collected. Then, gross observation, hematoxylin-eosin staining, and Masson staining were performed.

RESULTS

The bFGF/chitosan derivatives/collagen composite thermosensitive hydrogel groups and the control group had statistical differences in the relative alveolar bone height, relative epithelial down growth and grading count score of periodontal tissue regeneration (<0.05).

CONCLUSIONS

bFGF/chitosan derivatives/collagen composite thermosensitive hydrogels have good application prospects in periodontal tissue engineering.

摘要

目的

研究不同的壳聚糖衍生物热敏复合水凝胶作为牙周组织工程支架材料的可行性。

方法

制备了三种具有不同生物学特性的壳聚糖衍生物，即磺化壳聚糖（SCS）、磷酸化壳聚糖（PCS）和磷酸化磺化壳聚糖（PSCS）。使用碱性成纤维细胞生长因子（bFGF）、壳聚糖衍生物和胶原蛋白构建了三种热敏复合水凝胶。20 只雄性 Wistar 大鼠随机分为对照组、空白组、bFGF/SCS/胶原复合热敏水凝胶组、bFGF/PCS/胶原复合热敏水凝胶组和 bFGF/PSCS/胶原复合热敏水凝胶组。然后，建立三壁骨内缺损。用不同类型的热敏复合水凝胶处理缺陷。手术后 6 周处死动物，采集标本。然后进行大体观察、苏木精-伊红染色和 Masson 染色。