Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
Bachelor Program for Design and Materials for Medical Equipment and Devices, Da-Yeh University, Changhua, Taiwan; Orthopedic Department, Showchwan Memorial Hospital, Changhua, Taiwan.
Mater Sci Eng C Mater Biol Appl. 2020 Jul;112:110923. doi: 10.1016/j.msec.2020.110923. Epub 2020 Apr 5.
We report an efficient growth factor delivering system based on polypeptide/heparin composite hydrogels for wound healing application. Linear and star-shaped poly(l-lysine) (l-PLL and s-PLL) were chosen due to not only their cationic characteristics, facilitating the efficient complexation of negatively charged heparin, but also the ease to tune the physical and mechanical properties of as-prepared hydrogels simply by varying polypeptide topology and chain length. The results showed that polymer topology can be an additional parameter to tune hydrogel properties. Our experimental data showed that these composite hydrogels exhibited low hemolytic activity and good cell compatibility as well as excellent antibacterial activity, making them ideal as wound dressing materials. Unlike other heparin-based hydrogels, these composite hydrogels with heparin densely deposited on the surface can increase the stabilization and concentration of growth factor, which can facilitate the healing process as confirmed by our in vivo animal model. We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
我们报告了一种基于多肽/肝素复合水凝胶的高效生长因子传递系统，用于伤口愈合应用。线性和星形聚（L-赖氨酸）（l-PLL 和 s-PLL）被选择，不仅因为它们具有阳离子特性，有利于带负电荷的肝素的有效络合，而且还因为易于通过改变多肽拓扑和链长来简单地调节所制备水凝胶的物理和机械性能。结果表明，聚合物拓扑结构可以是调节水凝胶性能的附加参数。我们的实验数据表明，这些复合水凝胶具有低溶血活性、良好的细胞相容性和优异的抗菌活性，使其成为理想的伤口敷料材料。与其他基于肝素的水凝胶不同，这些表面上肝素密集沉积的复合水凝胶可以增加生长因子的稳定性和浓度，这可以通过我们的体内动物模型来促进愈合过程。我们相信这些 PLL/肝素复合水凝胶是有前途的伤口敷料材料，并且可能在其他生物医学领域有潜在的应用。
