用于口腔软组织增厚的丝素蛋白多孔支架的初步研究。
Preliminary study of silk fibroin porous scaffold for oral soft-tissue thickening.
作者信息
Li Dexiong, Cao Runyuan, Chen Jiang
机构信息
School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350000, China.
Institute of Stomatology & Research Center of Dental and Craniofacial Implants, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2022 Oct 1;40(5):513-521. doi: 10.7518/hxkq.2022.05.003.
OBJECTIVES
This study aimed to investigate the feasibility of three different concentrations of silk-fibroin porous scaffolds applied to oral soft-tissue thickening .
METHODS
Silk-fibroin scaffolds with three different concentrations (1 wt%, 3 wt%, and 5 wt%; denoted as SF1, SF3, and SF5, respectively) were prepared by freeze drying and methanol enhancement. The scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and thermogravimetric analysis. Pore size, porosity, and degradation rate were also evaluated. The three groups of scaffold materials (the experimental sides) and the collagen matrix (the control side) were implanted into the oral mucosa of New Zealand white rabbits. Changed in mucosa thickness before and 3 months after operation were compared. The metabolism and regeneration effect of each group were observed by histological hematoxylin-eosin (HE) and Masson staining.
RESULTS
SEM showed that the three groups of scaffolds were all cross-linked porous structures. XRD and FTIR showed that the three scaffolds were dominated by a relatively stable Silk Ⅱ structure, which degraded more slowly . Among them, SF3 had the largest pore size (133.40 μm±22.85 μm) and moderate porosity (90.05%±6.68%). results showed that the thickening effect of SF1 was similar to that of the control group because of insufficient space-maintenance property. Meanwhile, the properties of SF3 and SF5 were more stable, and the thickening effect was significantly better than those of the control group. However, unlike SF5 that induced obvious inflammation, SF3 showed better degradation, more fibrosis and angiogenesis, and less inflammatory response .
CONCLUSIONS
Silk-fibroin scaffolds can be applied to effectively thicken soft tissues, among which SF3 (3 wt%) silk fibroin scaffold exhibited the best physicochemical properties, histocompatibility, and mucosal-thickening effect.
目的
本研究旨在探讨三种不同浓度的丝素蛋白多孔支架应用于口腔软组织增厚的可行性。
方法
通过冷冻干燥和甲醇增强法制备三种不同浓度（1 wt%、3 wt%和5 wt%；分别记为SF1、SF3和SF5）的丝素蛋白支架。通过扫描电子显微镜（SEM）、傅里叶变换红外（FTIR）光谱、X射线衍射（XRD）和热重分析对支架进行表征。还评估了孔径、孔隙率和降解率。将三组支架材料（实验组）和胶原基质（对照组）植入新西兰白兔的口腔黏膜。比较手术前和术后3个月黏膜厚度的变化。通过组织学苏木精-伊红（HE）和Masson染色观察各组的代谢和再生效果。
结果
SEM显示三组支架均为交联多孔结构。XRD和FTIR显示三种支架均以相对稳定的丝素Ⅱ结构为主，降解较慢。其中，SF3的孔径最大（133.40μm±22.85μm），孔隙率适中（90.05%±6.68%）。结果显示，由于空间维持性能不足，SF1的增厚效果与对照组相似。同时，SF3和SF5的性能更稳定，增厚效果明显优于对照组。然而，与诱导明显炎症的SF5不同，SF3显示出更好的降解、更多的纤维化和血管生成，炎症反应更少。
结论
丝素蛋白支架可有效应用于软组织增厚，其中SF3（3 wt%）丝素蛋白支架表现出最佳的理化性质、组织相容性和黏膜增厚效果。
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