College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science & Technology, China Agricultural University, Haidian District, Beijing 100193, China.
Poult Sci. 2024 Jun;103(6):103670. doi: 10.1016/j.psj.2024.103670. Epub 2024 Mar 16.
Aging is associated with alterations in gut function, including intestinal inflammation, leaky gut, and impaired epithelial regeneration. Rejuvenating the aged gut is imperative to extend the laying cycle of aged laying hens. Genistein is known to have beneficial effects on age-related diseases, but its precise role in homeostasis of the aged gut of laying hens remains to be elucidated. In this study, 160 45-wk-old Hyline Brown laying hens were continuously fed a basal diet or a diet supplemented with 40 mg/kg genistein until they reached 100 wk of age. The results revealed that long-term genistein supplementation led to an improvement in the egg production rate and feed conversion ratio, as well as an increase in egg quality. Moreover, the expression levels of senescence markers, such as β-galactosidase, P16, and P21, were decreased in the gut of genistein-treated aged laying hens. Furthermore, genistein ameliorated gut dysfunctions, such as intestinal inflammation, leaky gut, and impaired epithelial regeneration. T cell-derived IL-10 plays a crucial role in the genistein-induced regulation of age-related intestinal inflammation. This study demonstrates that long-term consumption of genistein improves homeostasis in the aged gut and extends the laying cycle of aged laying hens. Moreover, the link between genistein and T cells provides a rationale for dietary intervention against age-associated gut dysfunction.
衰老是与肠道功能的改变有关，包括肠道炎症、肠漏和上皮细胞再生受损。恢复衰老肠道对于延长老龄蛋鸡的产蛋周期至关重要。染料木黄酮已知对与年龄相关的疾病有有益的影响，但它在老龄蛋鸡肠道内稳态中的确切作用仍有待阐明。在这项研究中，160 只 45 周龄的海兰褐蛋鸡连续喂食基础日粮或添加 40mg/kg 染料木黄酮的日粮，直到它们达到 100 周龄。结果表明，长期补充染料木黄酮可提高产蛋率和饲料转化率，并提高蛋品质。此外，染料木黄酮处理的老龄蛋鸡肠道中衰老标志物（如β-半乳糖苷酶、P16 和 P21）的表达水平降低。此外，染料木黄酮改善了肠道功能障碍，如肠道炎症、肠漏和上皮细胞再生受损。T 细胞衍生的 IL-10 在染料木黄酮诱导的与年龄相关的肠道炎症调节中发挥关键作用。本研究表明，长期食用染料木黄酮可改善老龄肠道内稳态并延长老龄蛋鸡的产蛋周期。此外，染料木黄酮与 T 细胞之间的联系为针对与年龄相关的肠道功能障碍的饮食干预提供了依据。
