Gong Haojie, Liang Fengjia, Cai Chunyan, Ding Xuemei, Bai Shiping, Zhang Keying, Zeng Qiufeng, Liu Yan, Xuan Yue, Xu Shengyu, Mao Xiangbing, Wang Jianping
Animal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, PR China.
Animal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, PR China.
Poult Sci. 2025 May;104(5):104979. doi: 10.1016/j.psj.2025.104979. Epub 2025 Mar 7.
Saccharomyces cerevisiae fermentation product (SCFP), a postbiotic feed additive, has potential to improve animal growth and productivity. However, its effects on post-peak laying hens have not been thoroughly investigated. Therefore, this study aimed to explore the effects of SCFP on production, egg quality, intestinal health, ovarian function, and cecal microbiota in post-peak laying hens. A total of 600 45-week-old Lohmann pink laying hens were randomly assigned into three treatments, with ten replicates and twenty hens per replicate. The hens were fed either a basal diet (CON) or basal diet supplemented with SCFP at 750 mg/kg (SCFP1) and 1250 mg/kg (SCFP2) for 16 weeks. The results showed no significant effects on the laying performance (P > 0.05). SCFP supplementation increased Haugh unit, yolk color, albumen height, and eggshell ratio compared to the CON diet(P < 0.05). Hens received SCFP diets exhibited a higher intestinal villus height-to-crypt depth ratio (P < 0.05) and up-regulated the expression of jejunal occludin, zonula occluden-1 (ZO-1), and mucin 2 (MUC-2) (P < 0.05). Additionally, SCFP supplementation increased the concentration of jejunal secretory immunoglobulin A (SIgA) (P < 0.05), elevated serum levels of immunoglobulin A (IgA), IgG, interleukin-10 (IL-10), and interferon-γ (IFN-γ) (P < 0.05). Furthermore, dietary SCFP tended to decrease ovarian cell apoptosis and enhanced antioxidant capacity in laying hens (P < 0.05). Compared to CON group, the SCFP1 and SCFP2 groups had lower total bacteria and Escherichia coli, higher Lactobacillus (P < 0.05), and a greater abundance of Streptococcus, Pedosphaerales, Christensenellales, and Prevotellaceae in cecum. Significant correlations were observed between egg quality, intestinal health, ovarian function, and cecal microbiota. In addition, cecal microbial functional prediction indicated that SCFP altered various nutritional metabolism pathways. Dietary SCFP supplementation effectively improved egg quality in post-peak laying hens by modulating intestinal health, ovarian function, and cecal microbiota. Collectively, SCFP could be used as a valuable feed additive for post-peak laying hens, with 1250 mg/kg SCFP showing the better effects.
酿酒酵母发酵产物（SCFP）作为一种后生元饲料添加剂，具有提高动物生长性能和生产性能的潜力。然而，其对产蛋后期母鸡的影响尚未得到充分研究。因此，本研究旨在探讨SCFP对产蛋后期母鸡生产性能、蛋品质、肠道健康、卵巢功能和盲肠微生物群的影响。总共600只45周龄的罗曼粉蛋鸡被随机分为三个处理组，每组10个重复，每个重复20只鸡。母鸡分别饲喂基础日粮（CON）或添加750 mg/kg（SCFP1）和1250 mg/kg（SCFP2）SCFP的基础日粮，为期16周。结果表明，对产蛋性能无显著影响（P>0.05）。与CON日粮相比，添加SCFP提高了哈氏单位、蛋黄颜色、蛋白高度和蛋壳比例（P<0.05）。采食SCFP日粮的母鸡肠道绒毛高度与隐窝深度比值更高（P<0.05），空肠闭合蛋白、闭合小环蛋白-1（ZO-1）和黏蛋白2（MUC-2）的表达上调（P<0.05）。此外，添加SCFP提高了空肠分泌型免疫球蛋白A（SIgA）的浓度（P<0.05），提高了血清免疫球蛋白A（IgA）、免疫球蛋白G（IgG）、白细胞介素-10（IL-10）和干扰素-γ（IFN-γ）水平（P<0.05）。此外，日粮SCFP倾向于减少产蛋后期母鸡的卵巢细胞凋亡并增强其抗氧化能力（P<0.05）。与CON组相比，SCFP1组和SCFP2组盲肠中的总细菌和大肠杆菌数量较低，乳酸菌数量较高（P<0.05），链球菌、 Pedosphaerales、 Christensenellales和普雷沃氏菌科的丰度更高。在蛋品质、肠道健康、卵巢功能和盲肠微生物群之间观察到显著相关性。此外，盲肠微生物功能预测表明，SCFP改变了各种营养代谢途径。日粮添加SCFP通过调节肠道健康、卵巢功能和盲肠微生物群，有效改善了产蛋后期母鸡的蛋品质。总体而言，SCFP可作为产蛋后期母鸡一种有价值的饲料添加剂，其中1250 mg/kg SCFP效果更佳。
