Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Islamic Republic of Iran.
Iran National Tumor Bank, Cancer Institute, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
Pathol Res Pract. 2024 May;257:155286. doi: 10.1016/j.prp.2024.155286. Epub 2024 Apr 1.
In spite of the decrease in breast cancer (BC) death rates, it has remained a significant public health concern. Dysregulation of the Hippo pathway contributes to breast cancer development and progression by enhancing cancerous cell proliferation, survival, invasion, and migration. Investigating the connection between specific lncRNAs (SNHG15, HCP5, and LINC01433) and YAP and WWTR1, and the impact of these lncRNAs on the expression of YAP and WWTR1 proteins in the Hippo pathway, may offer valuable understanding for BC diagnosis and treatment. Forty BC tissue samples were acquired from the Tumor Bank and utilized for RNA and protein extraction. Real-time PCR and western blotting techniques were performed to assess the gene and protein expressions, respectively. Correlations between variables and their associations with clinicopathological features in BC were evaluated using Mann-Whitney U or Student's t-test. Additionally, the analysis of the GEO database was utilized to validate the findings. In cancerous tissue, the up-regulation of YAP, WWTR1, HCP5, SNHG15, and Linc01433 at both the mRNA and protein levels corresponds to the findings in GEO datasets. A significant association was found between YAP and histological grade, while WWTR1 showed a correlation with family history and HER-2. The distinct and notable expression of YAP, WWTR1, SNHG15, HCP5, and Linc01433 in BC tissues, together with the results of combined ROC curve analysis derived from our finding and GEO database suggest that a combined panel of these 5 RNAs may have great potential in predicting of BC and its management.
尽管乳腺癌（BC）死亡率有所下降，但它仍然是一个重大的公共卫生关注点。 Hippo 通路的失调通过增强癌细胞的增殖、存活、侵袭和迁移，促进乳腺癌的发生和发展。研究特定 lncRNAs（SNHG15、HCP5 和 LINC01433）与 YAP 和 WWTR1 之间的联系，以及这些 lncRNAs 对 Hippo 通路中 YAP 和 WWTR1 蛋白表达的影响，可能为 BC 的诊断和治疗提供有价值的认识。从肿瘤库中获取了 40 个 BC 组织样本，并用于提取 RNA 和蛋白质。分别采用实时 PCR 和 Western blot 技术评估基因和蛋白表达。采用 Mann-Whitney U 或 Student's t 检验评估变量之间的相关性及其与 BC 临床病理特征的关联。此外，还利用 GEO 数据库进行了分析。在癌组织中，YAP、WWTR1、HCP5、SNHG15 和 Linc01433 的 mRNA 和蛋白水平上调与 GEO 数据集的结果一致。YAP 与组织学分级之间存在显著关联，而 WWTR1 与家族史和 HER-2 相关。YAP、WWTR1、SNHG15、HCP5 和 Linc01433 在 BC 组织中的独特显著表达，以及我们的发现和 GEO 数据库的联合 ROC 曲线分析结果表明，这些 5 个 RNA 的联合组合可能在预测 BC 及其管理方面具有很大的潜力。
