Department of Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, India; Department of Botany, Sri-Ganesh Rai P. G. College, Dobhi-Jaunpur, Uttar Pradesh, India.
Department of Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
Food Chem. 2024 Aug 15;449:139240. doi: 10.1016/j.foodchem.2024.139240. Epub 2024 Apr 4.
The study reports the efficacy of nanofabricated citronellal inside the chitosan biopolymer (NeCn) against Aspergillus flavus growth, aflatoxin B (AFB) production, and active ingredient biodeterioration (Piperine) in Piper longum L. The prepared NeCn was characterized by Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), and Fourier Transform Infrared Spectroscopy (FTIR). The results revealed that the NeCn exhibited distantly improved antifungal (1.25 μL/mL) and AFB inhibition (1.0 μL/mL) compared to free Cn. The perturbances in membrane function, mitochondrial membrane potential, antioxidant defense system, and regulatory genes (Ver-1 and Nor-1) of AFB biosynthesis were reported as probable modes of action of NeCn. The NeCn (1.25 μL/mL) effectively protects the P. longum from A. flavus (78.8%), AFB contamination (100%), and deterioration of Piperine (62.39%), thus demonstrating its potential as a promising novel antifungal agent for food preservation.
该研究报告了纳米柠檬醛在壳聚糖生物聚合物（NeCn）内对黄曲霉生长、黄曲霉毒素 B（AFB）产生和胡椒中活性成分（胡椒碱）生物降解的功效。制备的 NeCn 通过扫描电子显微镜（SEM）、动态光散射（DLS）和傅里叶变换红外光谱（FTIR）进行了表征。结果表明，与游离 Cn 相比，NeCn 表现出明显改善的抗真菌（1.25 μL/mL）和 AFB 抑制（1.0 μL/mL）作用。NeCn 的作用机制可能是扰乱膜功能、线粒体膜电位、抗氧化防御系统以及 AFB 生物合成的调节基因（Ver-1 和 Nor-1）。NeCn（1.25 μL/mL）可有效保护长胡椒免受黄曲霉（78.8%）、AFB 污染（100%）和胡椒碱恶化（62.39%）的侵害，因此证明其作为一种有前途的新型食品防腐剂具有潜力。
