Laboratory of Herbal Pesticides, Centre of Advanced Study (CAS) in Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, UP, India.
Laboratory of Herbal Pesticides, Centre of Advanced Study (CAS) in Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, UP, India.
Int J Biol Macromol. 2021 Feb 28;171:480-490. doi: 10.1016/j.ijbiomac.2021.01.024. Epub 2021 Jan 9.
In this study, a comparative efficacy of Cananga odorata EO (CoEO) and its nanoencapsulated formulation into chitosan nanoemulsion (CoEO-CsNe) against a toxigenic strain of Aspergillus flavus (AF-M-K5) were investigated for the first time in order to determine its efficacy in preservation of stored food from fungal, aflatoxin B (AFB) contamination and lipid peroxidation. GC and GC-MS analysis of CoEO revealed the presence of linalool (24.56%) and benzyl acetate (22.43%) as the major components. CoEO was encapsulated into chitosan nanoemulsion (CsNe) through ionic-gelation technique and characterized by High Resolution-Scanning Electron Microscopy (HR-SEM), Fourier Transform Infrared spectroscopy (FTIR), and X-Ray Diffraction (XRD) analysis. The CoEO-CsNe during in vitro investigation against A. flavus completely inhibited the growth and AFB production at 1.0 μL/mL and 0.75 μL/mL, respectively. Additionally, CoEO-CsNe showed improved antioxidant activity against DPPH and ABTS with IC value 0.93 and 0.72 μL/mL, respectively. Further, CoEO-CsNe suppressed fungal growth, AFB secretion and lipid peroxidation in Arachis hypogea L. during in situ investigation without causing any adverse effect on seed germination. Overall results demonstrated that the CoEO-CsNe has potential of being utilized as a suitable plant based antifungal agent to improve the shelf-life of stored food against AFB and lipid peroxidation mediated biodeterioration.
在这项研究中，首次研究了依兰依兰精油（CoEO）及其包封到壳聚糖纳米乳液中的纳米制剂（CoEO-CsNe）对产毒黄曲霉（AF-M-K5）的相对功效，以确定其在防止储存食品受到真菌、黄曲霉毒素 B（AFB）污染和脂质过氧化方面的功效。CoEO 的 GC 和 GC-MS 分析表明，其主要成分是芳樟醇（24.56%）和乙酸苄酯（22.43%）。CoEO 通过离子凝胶化技术包封到壳聚糖纳米乳液（CsNe）中，并通过高分辨率扫描电子显微镜（HR-SEM）、傅里叶变换红外光谱（FTIR）和 X 射线衍射（XRD）分析进行了表征。在体外研究中，CoEO-CsNe 在 1.0 μL/mL 和 0.75 μL/mL 时完全抑制了黄曲霉的生长和 AFB 的产生。此外，CoEO-CsNe 对 DPPH 和 ABTS 的抗氧化活性均有所提高，IC 值分别为 0.93 和 0.72 μL/mL。此外，CoEO-CsNe 在原位研究中抑制了花生（Arachis hypogea L.）中的真菌生长、AFB 分泌和脂质过氧化，而对种子发芽没有任何不良影响。总体结果表明，CoEO-CsNe 具有作为一种合适的植物源抗真菌剂的潜力，可提高储存食品的保质期，防止 AFB 和脂质过氧化介导的生物降解。
