Rmd9p 在酿酒酵母线粒体 15S rRNA 3′-末端加工中的作用。
Role of Rmd9p in 3'-end processing of mitochondrial 15S rRNA in Saccharomyces cerevisiae.
机构信息
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India.
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India; Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560064, India.
出版信息
Mitochondrion. 2024 May;76:101876. doi: 10.1016/j.mito.2024.101876. Epub 2024 Apr 8.
Ribosome biogenesis, involving processing/assembly of rRNAs and r-proteins is a vital process. In Saccharomyces cerevisiae mitochondria, ribosomal small subunit comprises 15S rRNA (15S). While the 15S 5'-end processing uses Ccm1p and Pet127p, the mechanisms of the 3'-end processing remain unclear. We reveal involvement of Rmd9p in safeguarding/processing 15S 3'-end. Rmd9p deficiency results in a cleavage at a position 183 nucleotides upstream of 15S 3'-end, and in the loss of the 3'-minor domain. Rmd9p binds to the sequences in the 3'-end region of 15S, and a genetic interaction between rmd9 and dss1 indicates that Rmd9p regulates/limits mtEXO activity during the 3'-end spacer processing.
核糖体生物发生，涉及 rRNA 和 r 蛋白的加工/组装，是一个至关重要的过程。在酿酒酵母线粒体中，核糖体小亚基由 15S rRNA（15S）组成。虽然 15S 的 5'端加工使用 Ccm1p 和 Pet127p，但 3'端加工的机制仍不清楚。我们揭示了 Rmd9p 在保护/加工 15S 3'端中的作用。Rmd9p 缺乏会导致在 15S 3'端上游 183 个核苷酸的位置发生切割，并且丧失 3'小域。Rmd9p 与 15S 的 3'端区域的序列结合，并且 rmd9 和 dss1 之间的遗传相互作用表明 Rmd9p 在 3'端间隔区加工过程中调节/限制 mtEXO 活性。
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