Department of Quality and Safety, Chinese Academy of Fishery Sciences, Beijing 100141, PR China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, Beijing 100141, PR China.
Department of Quality and Safety, Chinese Academy of Fishery Sciences, Beijing 100141, PR China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, Beijing 100141, PR China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, PR China.
Int J Biol Macromol. 2024 May;267(Pt 2):131188. doi: 10.1016/j.ijbiomac.2024.131188. Epub 2024 Apr 9.
Traditional spiking methods for preparing matrix reference material of aquatic products is difficult to control the drug content in the matrix, especially one matrix containing multiple drugs. Minced fish is commonly used for the preparation of matrix reference materials in aquatic products, which is a relatively complex matrix with stickiness and difficult handling. Drug loading capacity is a key factor affecting the effectiveness of matrix reference materials. Here, we proposed a new spiking approach to improve the drug loading capacity of seven quinolones based on microfluidics, simultaneously. Fresh grass carp tissue underwent grinding, fine filtration, centrifugation and reconstituted in distilled water to form a liquid sample, which was subsequently mixed with a sodium alginate solution (1 %) at a ratio of 1:1.2. The mixed solution was supplemented with seven quinolones of equal concentration, followed by the preparation of uniform fish gel microspheres using microfluidic technology. The results indicated that the recoveries of seven quinolones ranged from 82.54 % to 114.17 %, demonstrating a significant improvement in the drug loading capacity of these quinolones compared to traditional methods. Moreover, the drug concentration in the matrix can be precisely controlled. A strong linear relationship was observed between the concentration of seven quinolones in the matrix and its initial concentration, which could serve as a reference for the development of other matrix reference materials.
传统的水产基质标准物质制备方法采用的是 spike 法，难以控制基质中药物的含量，特别是一个基质中含有多种药物的情况。鱼糜是水产基质标准物质制备中常用的基质，其具有粘性，处理困难，基质较为复杂。载药量是影响基质标准物质效果的关键因素。在这里，我们提出了一种基于微流控技术的新的 spike 方法，同时提高了七种喹诺酮类药物的载药量。新鲜草鱼组织经过研磨、精细过滤、离心和重悬于蒸馏水中形成液体样品，然后与 1%（质量分数）的海藻酸钠溶液按 1:1.2 的比例混合。混合溶液中添加了浓度相等的七种喹诺酮类药物，然后使用微流控技术制备均匀的鱼凝胶微球。结果表明，七种喹诺酮类药物的回收率在 82.54%到 114.17%之间，与传统方法相比，这些喹诺酮类药物的载药量有了显著提高。此外，可以精确控制基质中的药物浓度。基质中七种喹诺酮类药物的浓度与其初始浓度之间存在很强的线性关系，可以为其他基质标准物质的开发提供参考。
