Wang Huijiao, Liu Huan, Sun Huiwu, Zhang Chaoying
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, P. R. China.
Department of Quality and Safety, Chinese Academy of Fishery Sciences, Beijing 100141, P. R. China.
ACS Omega. 2024 May 6;9(19):21459-21466. doi: 10.1021/acsomega.4c01874. eCollection 2024 May 14.
Medicated bath is the most common spiking method used in the development of matrix reference materials for aquatic products; however, the environmental issues caused by the treatment of waste liquid after medicated bath cannot be ignored. We proposed an environmentally friendly spiking method based on microfluidics, which significantly improved the drug utilization rate without the need for subsequent drug residue treatment. Finely processed minced fish samples were fully mixed with quinolone drugs, and minced fish gel microspheres were prepared by microfluidic technology, utilizing the gel's water-locking function to enhance the drug-loading capacity. The results showed that this method can significantly increase the drug-loading capacity of the matrix (2.33-4.03 times) compared with the traditional spiking methods. In addition, the matrix reference material prepared by this method has good stability, and the drug concentration was adjustable and controllable.
药浴是水产品基质标准物质研制中最常用的加标方法；然而，药浴后废液处理所引起的环境问题不容忽视。我们提出了一种基于微流控技术的环保型加标方法，该方法显著提高了药物利用率，且无需后续药物残留处理。将精细处理后的碎鱼样本与喹诺酮类药物充分混合，利用微流控技术制备碎鱼凝胶微球，借助凝胶的锁水功能提高载药量。结果表明，与传统加标方法相比，该方法可显著提高基质的载药量（2.33至4.03倍）。此外，用该方法制备的基质标准物质具有良好的稳定性，药物浓度可调节和控制。
