水稻 NARROW LEAF1 的催化三联体涉及 H234。
The catalytic triad of rice NARROW LEAF1 involves H234.
机构信息
College of Life Sciences, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, People's Republic of China.
School of Physical Science and Technology, Electron Microscopy Centre of Lanzhou University, Lanzhou University, Lanzhou, People's Republic of China.
出版信息
Nat Plants. 2024 May;10(5):743-748. doi: 10.1038/s41477-024-01668-1. Epub 2024 Apr 10.
NARROW LEAF1 (NAL1) exerts a multifaceted influence on leaf morphology and crop yield. Recent crystal study proposed that histidine 233 (H233) is part of the catalytic triad. Here we report that unlike suggested previously, H234 instead of H233 is a component of the catalytic triad alongside residues D291 and S385 in NAL1. Remarkably, residue 233 unexpectedly plays a pivotal role in regulating NAL1's proteolytic activity. These findings establish a strong foundation for utilizing NAL1 in breeding programs aimed at improving crop yield.
窄叶 1（NAL1）对叶片形态和作物产量有多种影响。最近的晶体研究提出组氨酸 233（H233）是催化三联体的一部分。在这里，我们报告说，与之前的建议不同，H234 而不是 H233 与 D291 和 S385 残基一起构成 NAL1 催化三联体的一部分。值得注意的是，残基 233 出人意料地在调节 NAL1 的蛋白水解活性中起着关键作用。这些发现为利用 NAL1 进行旨在提高作物产量的育种计划奠定了坚实的基础。
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