Stenman U H, Alfthan H, Koskimies A, Seppälä M, Pettersson K, Lövgren T
Ann N Y Acad Sci. 1985;442:544-50. doi: 10.1111/j.1749-6632.1985.tb37563.x.
A novel time-resolved immunofluorometric method is described for the estimation of human luteinizing hormone (LH) in serum and urine. The method utilizes two monoclonal antibodies: one reacting with the beta-subunit is adsorbed to the wall of a microtiter well, and the other is labeled with a fluorescent europium chelate and reacts with the alpha-subunit. The method is ultrarapid (15-30 min) and highly sensitive (1 IU/L). A large linear measuring range allows measurement of LH levels from 1 to 250 IU/L. For the monitoring of urinary LH, IFMA gives the same information as the Hi-Gonavis assay, but it has the advantages of greater sensitivity and a shorter assay time. For the determination of serum LH levels, an acceptable correlation was observed between radioimmunoassay and IFMA. Furthermore, IFMA has a greater sensitivity, and is more rapid and not dependent on the handling of radioactive materials.
本文描述了一种用于测定血清和尿液中人类促黄体生成素（LH）的新型时间分辨免疫荧光测定法。该方法使用两种单克隆抗体：一种与β亚基反应，吸附于微量滴定孔壁上，另一种用荧光铕螯合物标记，与α亚基反应。该方法超快速（15 - 30分钟）且高度灵敏（1 IU/L）。较大的线性测量范围允许测量1至250 IU/L的LH水平。对于监测尿LH，免疫荧光测定法（IFMA）与Hi - Gonavis检测法提供相同信息，但它具有更高灵敏度和更短检测时间的优点。对于测定血清LH水平，放射免疫测定法与IFMA之间观察到可接受的相关性。此外，IFMA具有更高的灵敏度，更快，且不依赖于放射性物质的处理。
