Department of Animal Sciences and Marine Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran.
J Anim Physiol Anim Nutr (Berl). 2024 Jul;108(4):1152-1163. doi: 10.1111/jpn.13961. Epub 2024 Apr 11.
As sturgeon breeding has proliferated, there has been a heightened demand for growth stimulators in their diets. This study aimed to determine the impact of dietary chitosan on growth performance, whole-body proximate composition, growth-related gene expression, and intestinal histology in juvenile Acipenser stellatus. A total of 180 A. stellatus juveniles with an average weight of 31.90 ± 0.73 g were fed with diets containing 0 (control), 1.5, 3.0, 4.5, and 6.0 g chitosan.kg basic diet for eight weeks. The findings revealed a significant enhancement in growth performance with rising chitosan concentrations. Furthermore, chitosan supplementation upregulated the expression of the growth hormone gene in both brain and liver tissues. In liver samples, the most pronounced expression of the insulin-like growth factor-1 gene was noted at 6.0 g chitosan.kg, while in brain samples, peak expressions were observed in both the 4.5 and 6.0 g chitosan.kg treatments. While the whole-body proximate composition remained relatively stable, there was a notable decrease in whole-body lipids with the escalation of chitosan dosage. Intestinal villi dimensions, both height and width, were amplified in the chitosan-supplemented groups compared to controls. In summation, chitosan supplementation showed promise in bolstering growth performance, refining intestinal morphology, and enhancing growth-related gene expression. Analysis of the polynomial regression of weight gain and specific growth rate revealed that the optimum dietary chitosan requirements in A. stellatus were 5.32 and 5.21 g chitosan.kg, respectively.
随着鲟鱼养殖的蓬勃发展，其饲料中对生长刺激剂的需求也日益增加。本研究旨在确定饲料壳聚糖对幼龄史氏鲟（Acipenser stellatus）生长性能、全鱼体成分、生长相关基因表达和肠道组织学的影响。共选择平均体重为 31.90±0.73g 的 180 尾史氏鲟幼鱼，投喂含 0（对照组）、1.5、3.0、4.5 和 6.0g 壳聚糖.kg 基础饲料的日粮 8 周。结果表明，随着壳聚糖浓度的升高，生长性能显著提高。此外，壳聚糖添加物可上调脑和肝组织中生长激素基因的表达。在肝组织中，胰岛素样生长因子-1 基因的表达在 6.0g 壳聚糖.kg 时最为显著，而在脑组织中，4.5 和 6.0g 壳聚糖.kg 处理时均呈现出峰值表达。尽管全鱼体成分相对稳定，但随着壳聚糖剂量的增加，全鱼体脂质含量显著下降。与对照组相比，添加壳聚糖组的肠道绒毛高度和宽度均增加。总之，壳聚糖添加物有望增强生长性能、改善肠道形态和增强生长相关基因的表达。体重增加和特定生长率的多项式回归分析表明，史氏鲟的最适饲料壳聚糖需求量分别为 5.32 和 5.21g 壳聚糖.kg。
