Wang Liansheng, Xu Hong, Wang Yang, Wang Chang'an, Li Jinnan, Zhao Zhigang, Luo Liang, Du Xue, Xu Qiyou
Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, China.
Fish Physiol Biochem. 2017 Jun;43(3):901-909. doi: 10.1007/s10695-017-0344-5. Epub 2017 Jan 23.
The objective of this study was to evaluate the effects of the supplementation of vitamin D on the growth, vitamin D metabolites, and osteocalcin secretion in juvenile Siberian sturgeon (Acipenser baerii). A 90-day growth trial was conducted with juvenile Siberian sturgeon (initial body weight 3.47 ± 0.14 g) fed seven isonitrogenous and isoenergetic practical diets (45% CP and 13% lipid) containing 60 (basal diet), 240, 450, 880, 1670, 3300, or 1.0 × 10 IU/kg feed (D60~D 1.0 × 10) vitamin D. The results showed that weight gain and specific growth rate increased as the dietary vitamin D levels increased from 450 to 3300 IU/kg (P < 0.05). The fish fed with D1670 and D3300 diets had higher crude lipid and ash levels than the fish fed the D60 diet (P < 0.05). The fish fed D880, D1670, or D3300 diets had higher 25-OH-D and 1,25-(OH)-D levels than the fish fed the D60 diet (P < 0.05). The fish fed D880, D1670, D3300, or D1.0 × 10 diets had higher osteocalcin levels than the fish fed the D60 diet (P < 0.05). Based on the broken line method analysis of weight gain and osteocalcin, the dietary vitamin D requirement of juvenile Siberian sturgeon was estimated to be 1683.30 and 1403.27 IU/kg per diet, respectively.
本研究的目的是评估补充维生素D对西伯利亚鲟幼鱼（Acipenser baerii）生长、维生素D代谢产物及骨钙素分泌的影响。以初始体重为3.47±0.14 g的西伯利亚鲟幼鱼进行了为期90天的生长试验，投喂七种等氮等能实用饲料（粗蛋白45%、脂肪13%），饲料中维生素D含量分别为60（基础饲料）、240、450、880、1670、3300或1.0×10 IU/kg饲料（D60~D1.0×10）。结果表明，随着饲料中维生素D水平从450增加到3300 IU/kg，增重和特定生长率升高（P<0.05）。投喂D1670和D3300饲料的鱼，其粗脂肪和灰分水平高于投喂D60饲料的鱼（P<0.05）。投喂D880、D1670或D3300饲料的鱼，其25-OH-D和1,25-(OH)₂-D水平高于投喂D60饲料的鱼（P<0.05）。投喂D880、D1670、D3300或D1.0×10饲料的鱼，其骨钙素水平高于投喂D60饲料的鱼（P<0.05）。基于增重和骨钙素的折线法分析，估计西伯利亚鲟幼鱼的饲料维生素D需求量分别为每千克饲料1683.30和1403.27 IU。
