Winsor B, Lacroute F, Ruet A, Sentenac A
Mol Gen Genet. 1979 Jun 7;173(2):145-51. doi: 10.1007/BF00330304.
Two hundred strains of Saccharomyces cerevisiae temperature sensitive for RNA synthesis were selected and screened in crude extracts for DNA-dependent RNA polymerase activities. One strain was isolated which had only residual in vitro RNA polymerase B activity. In normal growth conditions total RNA, poly A+ RNA and protein synthesis were indistinguishable from those of the wild type strain at 23 degrees C and after shift to 37 degrees C. A temperature sensitive phenotype was detected only when rpoB containing strains were grown in adverse conditions. The mutant character showed mendelian segregation and was coexpressed with the wild type character in heterozygous diploids. Residual enzyme activity was characterised in crude extracts using synthetic polymers and natural templates in different ionic conditions.
挑选出200株对RNA合成温度敏感的酿酒酵母菌株，并在粗提物中筛选其依赖DNA的RNA聚合酶活性。分离出一个菌株，该菌株仅具有残余的体外RNA聚合酶B活性。在正常生长条件下，总RNA、聚腺苷酸+RNA和蛋白质合成在23℃时与野生型菌株无异，转移至37℃后也是如此。只有当含有rpoB的菌株在不利条件下生长时，才检测到温度敏感表型。突变性状呈现孟德尔分离，并且在杂合二倍体中与野生型性状共表达。利用合成聚合物和天然模板在不同离子条件下对粗提物中的残余酶活性进行了表征。
