大鼠晶状体中的前列腺素生物合成。
Prostaglandin biosynthesis in the rat lens.
作者信息
Keeting P E, Lysz T W, Centra M, Fu S C
出版信息
Invest Ophthalmol Vis Sci. 1985 Aug;26(8):1083-6.
Rat lens microsomal preparations possess the capability of converting exogenous arachidonic acid into prostaglandins (PG) E2 and F2 alpha. The low, yet measurable prostaglandin biosynthetic capacity of the lens microsomes was demonstrated by radioimmunoassay (RIA) and by separation of radiolabeled products after incubation with high specific activity U-14 [C]-arachidonic acid. Maximal formation of radioimmunoassayable PGE2 and PGF2 alpha was measured after a 15-min incubation at 37 degrees C with approximately 0.5 mg protein. Prostaglandin biosynthesis was inhibited by the nonsteroidal anti-inflammatory agents aspirin (IC50 = 52 microM) and indomethacin (IC50 = 20 microM). These results unequivocally demonstrate prostaglandin biosynthesis in rat lens.
大鼠晶状体微粒体制剂具有将外源性花生四烯酸转化为前列腺素（PG）E2和F2α的能力。通过放射免疫测定法（RIA）以及在与高比活U-14[C]-花生四烯酸孵育后对放射性标记产物进行分离，证实了晶状体微粒体中前列腺素生物合成能力较低但可测量。在37℃下与约0.5mg蛋白质孵育15分钟后，测量到放射免疫可测定的PGE2和PGF2α的最大生成量。非甾体抗炎药阿司匹林（IC50 = 52μM）和吲哚美辛（IC50 = 20μM）可抑制前列腺素的生物合成。这些结果明确证明了大鼠晶状体中前列腺素的生物合成。
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