Blotman F, Chaintreuil J, Poubelle P, Flandre O, Crastes de Paulet A, Simon L
Adv Prostaglandin Thromboxane Res. 1980;8:1705-8.
Prostaglandins (PG) are currently suspected to be involved in human rheumatoid arthritis (RA). We have studied the PG [PGE2, PGF2 alpha, and Thromboxane B2 (TXB2)] biosynthesis capacity of normal (from amputations) and pathologic (osteoarthritis (OA) and RA) synovia. The measurement is done on the whole homogenate and microsomal fraction after 40 min incubation, without exogenous arachidonic acid (AA) or with a saturating concentration (25 micrograms/ml) of this compound, using a radioimmunoassay method. There is a considerable increase in PGE2 and PGF2 alpha production by the rheumatoid synovia and more PGE2 and PGF2 alpha are formed. This production is more marked for homogenates than microsomal fraction. The OA group is not homogeneous and does not differ significantly either from normals or from RA. The addition of AA considerably increases the biosynthesis of PG in both normal and pathologic tissue. For TXB2, the first results (four synovia) show no or a small biosynthesis in RA as well as in normals. Nevertheless, the levels before incubation seem higher in RA than in normal tissue.
目前怀疑前列腺素（PG）与人类类风湿性关节炎（RA）有关。我们研究了正常（截肢者）和病理（骨关节炎（OA）和RA）滑膜的PG [前列腺素E2（PGE2）、前列腺素F2α（PGF2α）和血栓素B2（TXB2）]生物合成能力。使用放射免疫分析法，在不添加外源性花生四烯酸（AA）或添加该化合物饱和浓度（25微克/毫升）的情况下，对整个匀浆和微粒体部分进行40分钟孵育后进行测量。类风湿滑膜产生的PGE2和PGF2α显著增加，且形成的PGE2和PGF2α更多。这种产生在匀浆中比在微粒体部分更明显。OA组不具有同质性，与正常组或RA组相比均无显著差异。添加AA可显著增加正常和病理组织中PG的生物合成。对于TXB2，初步结果（四个滑膜）显示RA和正常组织中均无或仅有少量生物合成。然而，孵育前RA组织中的水平似乎高于正常组织。
