School of Materials and Chemistry, University of Shanghai for Science and Technology, Shanghai 200093, China.
Department of Cardiology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China.
Biomacromolecules. 2024 May 13;25(5):3098-3111. doi: 10.1021/acs.biomac.4c00201. Epub 2024 Apr 12.
Biodegradable stents are the most promising alternatives for the treatment of cardiovascular disease nowadays, and the strategy of preparing functional coatings on the surface is highly anticipated for addressing adverse effects such as in-stent restenosis and stent thrombosis. Yet, inadequate mechanical stability and biomultifunctionality limit their clinical application. In this study, we developed a multicross-linking hydrogel on the polylactic acid substrates by dip coating that boasts impressive antithrombotic ability, antibacterial capability, mechanical stability, and self-healing ability. Gelatin methacryloyl, carboxymethyl chitosan, and oxidized sodium alginate construct a double-cross-linking hydrogel through the dynamic Schiff base chemical and in situ blue initiation reaction. Inspired by the adhesion mechanism employed by mussels, a triple-cross-linked hydrogel is formed with the addition of tannic acid to increase the adhesion and antibiofouling properties. The strength and hydrophilicity of hydrogel coating are regulated by changing the composition ratio and cross-linking degree. It has been demonstrated in tests in vitro that the hydrogel coating significantly reduces the adhesion of proteins, MC3T3-E1 cells, platelets, and bacteria by 85% and minimizes the formation of blood clots. The hydrogel coating also exhibits excellent antimicrobial in vitro and antiinflammatory properties in vivo, indicating its potential value in vascular intervention and other biomedical fields.
可生物降解支架是当今治疗心血管疾病最有前途的替代品，在表面制备功能涂层的策略备受期待，可以解决支架内再狭窄和支架内血栓形成等不良反应。然而，其机械稳定性和生物多功能性不足限制了其临床应用。在本研究中，我们通过浸涂在聚乳酸基底上开发了一种多交联水凝胶，具有令人印象深刻的抗血栓形成能力、抗菌能力、机械稳定性和自修复能力。明胶甲基丙烯酰、羧甲基壳聚糖和氧化海藻酸钠通过动态席夫碱化学和原位蓝色引发反应构建双交联水凝胶。受贻贝所采用的粘附机制的启发，通过添加单宁酸形成了三交联水凝胶，以提高粘附性和抗生物污损性。通过改变组成比和交联度来调节水凝胶涂层的强度和亲水性。体外试验表明，水凝胶涂层可使蛋白质、MC3T3-E1 细胞、血小板和细菌的粘附减少 85%，并最大限度地减少血栓形成。水凝胶涂层还表现出优异的体外抗菌和体内抗炎性能，表明其在血管介入和其他生物医学领域具有潜在价值。
