Aglietta M, Piacibello W, Stacchini A, Sanavio F, Gavosto F
Leuk Res. 1985;9(7):879-83. doi: 10.1016/0145-2126(85)90309-1.
The effect of increasing concentrations of retinoic acid (RA) on the in-vitro proliferation of normal and chronic myeloid leukemia (CML) granulo-monocyte precursors (CFU-GM) was studied. 10(-7)M RA added to semisolid cultures stimulated the growth of day 14 but not of day 7 normal CFU-GM, whereas in CML the growth of both populations was either unchanged or inhibited. Five-day and 10-day preincubation of normal bone marrow cells with RA augmented the number of day 14 CFU-GM (by up to 187% with 10(-6) M RA), whereas there was a marked decrease when CML cells were used. Total cellularity was not much affected, though a slight increase in liquid normal bone marrow cultures and a slight fall in CML cultures could be detected. These data point to a difference in the response to RA of normal and CML precursors. They may offer of preclinical basis for its employment to delay the blastic progression of CML.
研究了不同浓度视黄酸（RA）对正常及慢性髓性白血病（CML）粒-单核细胞前体（CFU-GM）体外增殖的影响。向半固体培养基中添加10^(-7)M RA可刺激第14天正常CFU-GM的生长，但对第7天的正常CFU-GM无刺激作用；而在CML中，这两个群体的生长要么没有变化，要么受到抑制。正常骨髓细胞与RA预孵育5天和10天可增加第14天CFU-GM的数量（10^(-6)M RA时增加高达187%），而使用CML细胞时则显著减少。总细胞数受影响不大，不过在液体正常骨髓培养中可检测到轻微增加，在CML培养中则有轻微下降。这些数据表明正常和CML前体对RA的反应存在差异。它们可能为使用RA延缓CML的急变进展提供临床前依据。
