• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

稳态和脉冲电化学再生 1,4-NADH 的产物分布及与酶反应的集成。

Product Distribution of Steady-State and Pulsed Electrochemical Regeneration of 1,4-NADH and Integration with Enzymatic Reaction.

机构信息

Electrochemical Energy Conversion, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstraße 1, 39106, Magdeburg, Germany.

Institute of Biochemistry, University of Greifswald, Felix-Hausdorff-Str. 4, 17487, Greifswald, Germany.

出版信息

ChemistryOpen. 2024 Aug;13(8):e202400064. doi: 10.1002/open.202400064. Epub 2024 Apr 12.

DOI:10.1002/open.202400064
PMID:38607952
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11319214/
Abstract

The direct electrochemical reduction of nicotinamide adenine dinucleotide (NAD) results in various products, complicating the regeneration of the crucial 1,4-NADH cofactor for enzymatic reactions. Previous research primarily focused on steady-state polarization to examine potential impacts on product selectivity. However, this study explores the influence of dynamic conditions on the selectivity of NAD reduction products by comparing two dynamic profiles with steady-state conditions. Our findings reveal that the main products, including 1,4-NADH, several dimers, and ADP-ribose, remained consistent across all conditions. A minor by-product, 1,6-NADH, was also identified. The product distribution varied depending on the experimental conditions (steady state vs. dynamic) and the concentration of NAD, with higher concentrations and overpotentials promoting dimerization. The optimal yield of 1,4-NADH was achieved under steady-state conditions with low overpotential and NAD concentrations. While dynamic conditions enhanced the 1,4-NADH yield at shorter reaction times, they also resulted in a significant amount of unidentified products. Furthermore, this study assessed the potential of using pulsed electrochemical regeneration of 1,4-NADH with enoate reductase (XenB) for cyclohexenone reduction.

摘要

烟酰胺腺嘌呤二核苷酸（NAD）的直接电化学还原会产生各种产物，这使得酶反应中关键的 1,4-NADH 辅酶的再生变得复杂。以前的研究主要集中在稳态极化上，以研究其对产物选择性的潜在影响。然而，本研究通过比较两种稳态条件下的动态曲线来探究动态条件对 NAD 还原产物选择性的影响。我们的研究结果表明，包括 1,4-NADH、几种二聚体和 ADP-核糖在内的主要产物在所有条件下保持一致。还鉴定出一种次要的副产物 1,6-NADH。产物分布取决于实验条件（稳态与动态）和 NAD 浓度，较高的浓度和过电势促进二聚化。在低过电势和 NAD 浓度下，通过稳态条件可实现 1,4-NADH 的最佳产量。虽然动态条件在较短的反应时间内提高了 1,4-NADH 的产率，但也导致了大量未识别的产物。此外，本研究评估了使用烯醇还原酶（XenB）进行脉冲电化学 1,4-NADH 再生用于环己烯酮还原的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/ed10c8bde2db/OPEN-13-e202400064-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/99bd8aef6031/OPEN-13-e202400064-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/433aabf7c9e5/OPEN-13-e202400064-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/e2bbf3faf811/OPEN-13-e202400064-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/2ac759e90878/OPEN-13-e202400064-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/4a1ea9692872/OPEN-13-e202400064-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/98d7db035edf/OPEN-13-e202400064-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/d52612e97ddf/OPEN-13-e202400064-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/ed10c8bde2db/OPEN-13-e202400064-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/99bd8aef6031/OPEN-13-e202400064-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/433aabf7c9e5/OPEN-13-e202400064-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/e2bbf3faf811/OPEN-13-e202400064-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/2ac759e90878/OPEN-13-e202400064-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/4a1ea9692872/OPEN-13-e202400064-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/98d7db035edf/OPEN-13-e202400064-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/d52612e97ddf/OPEN-13-e202400064-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad01/11319214/ed10c8bde2db/OPEN-13-e202400064-g007.jpg

相似文献

1
Product Distribution of Steady-State and Pulsed Electrochemical Regeneration of 1,4-NADH and Integration with Enzymatic Reaction.稳态和脉冲电化学再生 1,4-NADH 的产物分布及与酶反应的集成。
ChemistryOpen. 2024 Aug;13(8):e202400064. doi: 10.1002/open.202400064. Epub 2024 Apr 12.
2
Recent Progress and Perspectives on Electrochemical Regeneration of Reduced Nicotinamide Adenine Dinucleotide (NADH).电化学再生还原型烟酰胺腺嘌呤二核苷酸（NADH）的最新进展和展望。
Chem Asian J. 2020 Dec 14;15(24):4256-4270. doi: 10.1002/asia.202001035. Epub 2020 Nov 19.
3
Improved strategies for electrochemical 1,4-NAD(P)H regeneration: A new era of bioreactors for industrial biocatalysis.电化学 1,4-NAD(P)H 再生的改进策略：工业生物催化用生物反应器的新纪元。
Biotechnol Adv. 2018 Jan-Feb;36(1):120-131. doi: 10.1016/j.biotechadv.2017.10.003. Epub 2017 Oct 10.
4
Metabolic engineering of Escherichia coli: increase of NADH availability by overexpressing an NAD(+)-dependent formate dehydrogenase.大肠杆菌的代谢工程：通过过表达一种依赖NAD⁺的甲酸脱氢酶提高NADH的可用性。
Metab Eng. 2002 Jul;4(3):217-29. doi: 10.1006/mben.2002.0227.
5
Bioorganometallic chemistry. 13. Regioselective reduction of NAD(+) models, 1-benzylnicotinamde triflate and beta-nicotinamide ribose-5'-methyl phosphate, with in situ generated [CpRh(Bpy)H](+): structure-activity relationships, kinetics, and mechanistic aspects in the formation of the 1,4-NADH derivatives.生物有机金属化学。13. 用原位生成的[CpRh(Bpy)H](+)对NAD(+)模型、1-苄基烟酰胺三氟甲磺酸盐和β-烟酰胺核糖-5'-甲基磷酸进行区域选择性还原：1,4-NADH衍生物形成过程中的构效关系、动力学及机理方面
Inorg Chem. 2001 Dec 17;40(26):6705-16. doi: 10.1021/ic010562z.
6
Nicotinamide adenine dinucleotide hydrogen regeneration in a microbial electrosynthesis system by Enterobacter aerogenes.肠杆菌属 Aerogenes 在微生物电合成系统中通过烟酰胺腺嘌呤二核苷酸氢再生。
Bioelectrochemistry. 2023 Feb;149:108309. doi: 10.1016/j.bioelechem.2022.108309. Epub 2022 Oct 20.
7
Influence of electrode potential, pH and NAD concentration on the electrochemical NADH regeneration.电极电位、pH值和NAD浓度对电化学NADH再生的影响。
Sci Rep. 2022 Sep 30;12(1):16380. doi: 10.1038/s41598-022-20508-w.
8
Bioelectrocatalysis with a palladium membrane reactor.钯膜反应器中的生物电化学催化。
Nat Commun. 2023 Mar 31;14(1):1814. doi: 10.1038/s41467-023-37257-7.
9
Effect of culture conditions on the NADH/NAD ratio and total amounts of NAD(H) in chemostat cultures of Enterococcus faecalis NCTC 775.培养条件对粪肠球菌NCTC 775恒化器培养物中NADH/NAD比率及NAD(H)总量的影响
FEMS Microbiol Lett. 1994 Mar 1;116(3):263-7. doi: 10.1111/j.1574-6968.1994.tb06713.x.
10
Determinants of brain activation-induced cortical NAD/NADH responses in vivo.体内脑激活诱导的皮质NAD/NADH反应的决定因素。
Brain Res. 1984 May 7;299(1):61-72. doi: 10.1016/0006-8993(84)90788-1.

本文引用的文献

1
Engineering a Formate Dehydrogenase for NADPH Regeneration.构建用于NADPH再生的甲酸脱氢酶。
Chembiochem. 2023 Oct 17;24(20):e202300390. doi: 10.1002/cbic.202300390. Epub 2023 Aug 28.
2
Pulsed electrolysis - explained.脉冲电解——解析
Faraday Discuss. 2023 Oct 12;246(0):179-197. doi: 10.1039/d3fd00030c.
3
Discovery and Characterization of a Baeyer-Villiger Monooxygenase Using Sequence Similarity Network Analysis.利用序列相似性网络分析发现并鉴定一种Baeyer-Villiger单加氧酶
Chembiochem. 2023 May 16;24(10):e202200746. doi: 10.1002/cbic.202200746. Epub 2023 Apr 27.
4
Electrocatalytic NAD reduction hydrogen atom-coupled electron transfer.电催化NAD还原：氢原子耦合电子转移
Chem Sci. 2022 Oct 24;13(45):13361-13367. doi: 10.1039/d2sc02691k. eCollection 2022 Nov 23.
5
Influence of electrode potential, pH and NAD concentration on the electrochemical NADH regeneration.电极电位、pH值和NAD浓度对电化学NADH再生的影响。
Sci Rep. 2022 Sep 30;12(1):16380. doi: 10.1038/s41598-022-20508-w.
6
Selectivity in Electrochemical CO Reduction.电化学 CO 还原的选择性。
Acc Chem Res. 2022 Jan 18;55(2):134-144. doi: 10.1021/acs.accounts.1c00678. Epub 2022 Jan 6.
7
Enzyme nomenclature and classification: the state of the art.酶命名法和分类：现状。
FEBS J. 2023 May;290(9):2214-2231. doi: 10.1111/febs.16274. Epub 2022 Jan 3.
8
Bioelectrocatalytic Cofactor Regeneration Coupled to CO Fixation in a Redox-Active Hydrogel for Stereoselective C-C Bond Formation.在氧化还原活性水凝胶中进行生物电化学辅助辅酶再生与 CO2 固定以用于立体选择性 C-C 键形成。
Angew Chem Int Ed Engl. 2021 Sep 13;60(38):21056-21061. doi: 10.1002/anie.202103634. Epub 2021 Jul 7.
9
Recent Progress and Perspectives on Electrochemical Regeneration of Reduced Nicotinamide Adenine Dinucleotide (NADH).电化学再生还原型烟酰胺腺嘌呤二核苷酸（NADH）的最新进展和展望。
Chem Asian J. 2020 Dec 14;15(24):4256-4270. doi: 10.1002/asia.202001035. Epub 2020 Nov 19.
10
Biocatalysis: Enzymatic Synthesis for Industrial Applications.生物催化：工业应用中的酶法合成。
Angew Chem Int Ed Engl. 2021 Jan 4;60(1):88-119. doi: 10.1002/anie.202006648. Epub 2020 Aug 17.