Department of Biotechnology and Microbiology, Faculty of Chemistry, Gdańsk University of Technology, ul. Narutowicza 11/12, Gdańsk 80-233, Poland.
Department of Applied Physics, Faculty of Sciences, University of Granada, Campus de Fuentenueva sn, 18071 Granada, Spain.
Food Res Int. 2024 May;184:114255. doi: 10.1016/j.foodres.2024.114255. Epub 2024 Mar 19.
Bile Salts (BS) are responsible for stimulating lipid digestion in our organism. Gut microbiota are responsible for the deconjugation process of primary conjugated to secondary unconjugated BS. We use two structurally distinct BS and characterize the rate of lipolysis as a compound parameter. A static in-vitro digestion model as well as meta-analysis of literature data has been performed to determine the most influential factors affecting the lipid digestion process. The results demonstrate that lipolysis of emulsions using conjugated BS (NaTC, FFA = 60.0 %, CMC in SIF = 5.58 mM, MSR of linoleic acid = 0.21, rate of adsorption = -0.057 mN/m.s) enhances the release of FFA compared to deconjugated BS (NaDC, FFA = 49.5 %, CMC in SIF = 2.49 mM, MSR of linoleic acid = 0.16 rate of adsorption = -0.064 mN/m.s). These results indicate that conjugation plays an important role in controlling the rate of lipolysis in our organism which can be in turn, tuned by the microflora composition of our gut, ultimately controlling the rate of deconjugation of the BS.
胆汁盐（BS）负责刺激我们体内的脂质消化。肠道微生物群负责初级共轭 BS 向次级非共轭 BS 的去共轭过程。我们使用两种结构上不同的 BS，并将脂肪分解率作为一个复合参数进行特征描述。通过静态体外消化模型和文献数据的荟萃分析，确定了影响脂质消化过程的最主要影响因素。结果表明，与去共轭 BS（NaDC，FFA=49.5%，SIF 中的 CMC=2.49mM，亚油酸 MSR=0.16，吸附速率=-0.064 mN/m.s）相比，使用共轭 BS（NaTC，FFA=60.0%，SIF 中的 CMC=5.58mM，亚油酸 MSR=0.21，吸附速率=-0.057 mN/m.s）乳化剂增强了 FFA 的释放。这些结果表明，共轭在控制我们体内脂肪分解率方面起着重要作用，而脂肪分解率又可以通过我们肠道微生物群的组成进行调节，最终控制 BS 的去共轭速率。
