School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.
School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.
Anal Chim Acta. 2024 May 15;1303:342504. doi: 10.1016/j.aca.2024.342504. Epub 2024 Mar 23.
Food safety has always been a great concern, and the detection of additives is vital to ensuring food safety. Therefore, there is a necessity to develop a method that can quickly and efficiently separate and detect additives in food. High performance liquid chromatography is widely used in the analysis and testing of food additives. Ionic liquids have attracted wide attention in the preparation of high performance liquid chromatography stationary phases owing to their high stability, low vapor pressure and adjustable structure.
We developed a novel dicationic imidazole ionic liquid stationary phase for the simultaneous determination of organic preservatives (sodium benzoate, potassium sorbate) and inorganic preservatives (nitrate and nitrite) in foodstuffs under mixed-mode chromatography. The method had the advantages of easy operation, high reproducibility, good linearity and precision. In the detection of these four preservatives, the limit of detection ≤0.4740 mg⋅L and the limit of quantification ≤1.5800 mg⋅L. The intra-day and inter-day precision were less than 4.02%, and the recovery rate was 95.90∼100.19 %. At the same time, we also characterized the stationary phase, explored the mechanism and evaluated the chromatographic performance. The stationary phase was able to operate under the mixed mode of reversed phase/hydrophilic interaction/ion exchange chromatography, and it was capable of separating hydrophilic substances, hydrophobic substances, acids, and inorganic anionic substances with good separation efficiency and had high column efficiency.
In summary, the stationary phase has a promising application in the routine analysis of organic and inorganic preservatives in food. In addition, the stationary phase has good separation ability for hydrophilic, hydrophobic, ionic substances and complex samples, making it a prospective material for chromatographic separation.
食品安全一直是人们关注的焦点，添加剂的检测对于保障食品安全至关重要。因此，有必要开发一种能够快速、高效地分离和检测食品中添加剂的方法。高效液相色谱法在食品添加剂的分析和检测中得到了广泛的应用。离子液体因其高稳定性、低蒸气压和可调节的结构，在制备高效液相色谱固定相方面引起了广泛的关注。
我们开发了一种新型双阳离子咪唑离子液体固定相，用于混合模式色谱法同时测定食品中的有机防腐剂（苯甲酸钠、山梨酸钾）和无机防腐剂（硝酸盐和亚硝酸盐）。该方法具有操作简单、重现性好、线性和精密度好等优点。在检测这四种防腐剂时，检出限≤0.4740mg·L，定量限≤1.5800mg·L。日内和日间精密度均小于 4.02%，回收率为 95.90%~100.19%。同时，我们还对固定相进行了表征，探讨了其作用机制，评价了其色谱性能。该固定相能够在反相/亲水相互作用/离子交换色谱的混合模式下工作，对亲水性物质、疏水性物质、酸和无机阴离子物质具有良好的分离效率和较高的柱效。
综上所述，该固定相有望用于食品中有机和无机防腐剂的常规分析。此外，该固定相对亲水性、疏水性、离子性物质和复杂样品具有良好的分离能力，是一种有前途的色谱分离材料。
