采用2A核糖体跳跃原理追踪辣椒轻斑驳病毒的组装病毒粒子 于……（原文此处不完整）
Adoption of the 2A Ribosomal Skip Principle to Track Assembled Virions of Pepper Mild Mottle Virus in .
作者信息
Jiao Mengting, Yin Yueyan, Tian Yanzhen, Lei Jianing, Lin Lin, Wu Jian, Lu Yuwen, Zheng Hongying, Yan Fei, Wang Jianguang, Peng Jiejun
机构信息
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
出版信息
Plants (Basel). 2024 Mar 22;13(7):928. doi: 10.3390/plants13070928.
The coat protein (CP) is an important structural protein that plays many functional roles during the viral cycle. In this study, the CP of pepper mild mottle virus (PMMoV) was genetically fused to GFP using the foot-and-mouth disease virus peptide 2A linker peptide and the construct (PMMoV-GFP) was shown to be infectious. The systemic spread of the virus was monitored by its fluorescence in infected plants. Electron microscopy and immunocolloidal gold labelling confirmed that PMMoV-GFP forms rod-shaped particles on which GFP is displayed. Studies of tissue ultrastructure and virion self-assembly confirmed that PMMoV-GFP could be used to monitor the real-time dynamic changes of CP location during virus infection. Aggregations of GFP-tagged virions appeared as fluorescent plaques in confocal laser microscopy. Altogether, PMMoV-GFP is a useful tool for studying the spatial and temporal changes of PMMoV CP during viral infection.
外壳蛋白（CP）是一种重要的结构蛋白，在病毒循环过程中发挥多种功能作用。在本研究中，利用口蹄疫病毒肽2A连接肽将辣椒轻斑驳病毒（PMMoV）的CP与绿色荧光蛋白（GFP）进行基因融合，构建体（PMMoV-GFP）被证明具有感染性。通过感染植物中的荧光监测病毒的系统传播。电子显微镜和免疫胶体金标记证实PMMoV-GFP形成杆状颗粒，GFP展示在其上。组织超微结构和病毒粒子自组装研究证实PMMoV-GFP可用于监测病毒感染期间CP位置的实时动态变化。在共聚焦激光显微镜下，带有GFP标签的病毒粒子聚集体呈现为荧光斑块。总之，PMMoV-GFP是研究病毒感染期间PMMoV CP时空变化的有用工具。
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