González-Espíndola Luis Ángel, Pedroza-Sandoval Aurelio, Trejo-Calzada Ricardo, Jacobo-Salcedo María Del Rosario, García de Los Santos Gabino, Quezada-Rivera Jesús Josafath
Universidad Autónoma Chapingo, Unidad Regional Universitaria de Zonas Áridas, Km 40 Carretera Gómez Palacio-Chihuahua, Bermejillo C.P. 35230, Durango, Mexico.
Colegio de Postgraduados, Campus Montecillo, Km 36.5 Carretera México-Texcoco, Montecillo C.P. 56230, Texcoco, Mexico.
Plants (Basel). 2024 Mar 26;13(7):961. doi: 10.3390/plants13070961.
This study aimed to evaluate different L. ecotypes under water-deficit conditions to identify changes in relative water content and photosynthetic pigments as indicators of physiological responses during different years' seasons. The experiment was conducted in a randomized block design with three replicates. Ten treatments were performed as a factorial of 2 × 5, where the first variation factor was the soil water content-no water deficit (NDW) with 100% field capacity (FC), and water deficit (DW) corresponding to 85.4% of the FC-and the second variation factor comprised four ecotypes and one variety of . A significant effect was identified on the concentration of photosynthetic pigments, mainly total chlorophyll, with chlorophyll a in the 255301 ecotype with records of 187.8, 167.5, and 194.6 mg g FW in WD, corresponding to an increase of 86.0%, 172.6%, and 16.6%, respectively, in relation the lower values obtained in the ecotype 202700 under NWD. In carotenoids, higher concentrations were observed in the 255301 and 202700 ecotypes and the Estanzuela Ganador variety under WD in most seasonal periods, except summer; a similar response was found in the 202700 ecotype and the Estanzuela Ganador variety during the winter season, also in WD. The results showed that the first two principal components accounted for 71.8% of the total variation, with PC1 representing chlorophyll , chlorophyll , and total chlorophyll, and PC2 representing carotenoids, temperature, relative chlorophyll index, and relative water content. The observations were grouped based on soil moisture content, with the optimal moisture group exhibiting higher chlorophyll and carotenoid concentrations. The findings suggest that soil moisture content significantly affects the performance of ecotypes, and the plant shows seasonal variations in response to water-deficit conditions. This research contributes to understanding the physiological responses of and its potential as a water-efficient forage crop for promoting sustainable agriculture and enhancing food security.
本研究旨在评估水分亏缺条件下不同的披碱草生态型，以确定相对含水量和光合色素的变化，作为不同年份季节生理反应的指标。试验采用随机区组设计，重复三次。进行了10种处理，为2×5析因试验，其中第一个变异因素是土壤含水量——无水分亏缺（NDW），田间持水量（FC）为100%，以及对应于FC的85.4%的水分亏缺（DW）；第二个变异因素包括四种生态型和一种披碱草品种。结果发现，光合色素浓度，主要是总叶绿素，有显著影响，在255301生态型中叶绿素a在水分亏缺（WD）时的记录分别为187.8、167.5和194.6 mg g FW，相对于在无水分亏缺（NWD）条件下202700生态型获得的较低值，分别增加了86.0%、172.6%和16.6%。在类胡萝卜素方面，除夏季外，在大多数季节中，255301和202700生态型以及埃斯坦祖埃拉·加纳德品种在水分亏缺（WD）时观察到较高浓度；在冬季，202700生态型和埃斯坦祖埃拉·加纳德品种在水分亏缺（WD）时也有类似反应。结果表明，前两个主成分占总变异的71.8%，PC1代表叶绿素a、叶绿素b和总叶绿素，PC2代表类胡萝卜素、温度、相对叶绿素指数和相对含水量。观察结果根据土壤湿度含量进行分组，最佳湿度组表现出较高的叶绿素和类胡萝卜素浓度。研究结果表明，土壤湿度含量显著影响披碱草生态型的表现，并且植物在对水分亏缺条件的响应中表现出季节性变化。本研究有助于了解披碱草的生理反应及其作为节水型饲料作物促进可持续农业和加强粮食安全的潜力。
