Park Tae-Seon, Min Dong-Joo, Park Ji-Soo, Hong Jin-Sung
Interdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon 24341, Republic of Korea.
Plants (Basel). 2024 Mar 31;13(7):1001. doi: 10.3390/plants13071001.
belongs to the family and is used as both an oilseed and vegetable crop. As only a few studies have reported on the cucumber mosaic virus (CMV) in , we conducted this study to provide a basic understanding of the and CMV interactions. -infecting CMV (CMV-Co6) and non-infecting CMV (CMV-Rs1) were used. To identify the determinants of systemic infection in , we first constructed infectious clones of CMV-Co6 and CMV-Rs1 and used them as pseudo-recombinants. RNA2 of CMV was identified as an important determinant in because were systemically infected with RNA2-containing pseudo-recombinants; CMV-Co6, R/6/R, and R/6/6 were systemically infected . Subsequently, the amino acids of the 2a and 2b proteins were compared, and a chimeric clone was constructed. The chimeric virus R/6Rns/R6cp, containing the C-terminal region of the 2a protein of CMV-Rs1, still infects . It is the 2a protein that determines the systemic CMV infection in , suggesting that conserved 160G and 214A may play a role in systemic CMV infection in .
属于该科，用作油料作物和蔬菜作物。由于仅有少数研究报道了[具体作物名称]中的黄瓜花叶病毒（CMV），我们开展了本研究以初步了解[具体作物名称]与CMV的相互作用。使用了感染[具体作物名称]的CMV（CMV-Co6）和不感染[具体作物名称]的CMV（CMV-Rs1）。为了确定[具体作物名称]中系统感染的决定因素，我们首先构建了CMV-Co6和CMV-Rs1的感染性克隆，并将它们用作假重组体。CMV的RNA2被确定为[具体作物名称]中系统感染的一个重要决定因素，因为[具体作物名称]被含RNA2的假重组体系统感染；CMV-Co6、R/6/R和R/6/6被系统感染。随后，比较了2a和2b蛋白的氨基酸，并构建了一个嵌合克隆。含有CMV-Rs1 2a蛋白C末端区域的嵌合病毒R/6Rns/R6cp仍能感染[具体作物名称]。是2a蛋白决定了[具体作物名称]中CMV的系统感染，这表明保守的160G和214A可能在[具体作物名称]中CMV的系统感染中起作用。
