Takeshita M, Suzuki M, Kuwata S, Takanami Y
Laboratory of Plant Pathology, Kyushu University, Fukuoka, Japan.
Arch Virol. 1998;143(6):1109-17. doi: 10.1007/s007050050359.
The genetics of cucumber mosaic cucumovirus (CMV) and the pathogenicity of the virus for Raphanus sativus were analyzed using pseudorecombinants constructed from the infectious transcripts of two naturally occurring strains of cucumber mosaic cucumovirus (CMV-D8 and CMV-Y). CMV-D8, but not CMV-Y, could cause systemic infection of the plant. Viral accumulation and systemic movement in the plants was examined using immuno-tissue blot analysis, dot blot and Northern blot hybridization. Virus was equally distributed and CMV RNAs accumulated to similar levels in the inoculated cotyledons of radish irrespective of the pseudorecombinant, suggesting that there are no apparent differences in the ability of infection and viral accumulation between CMV-D8 and CMV-Y. We found, however, that both RNAs 2 and 3 of CMV-D8 are involved in determining the efficiency for the systemic infection of R. sativus. Co-operated interactions between genetic information of RNAs 2 and 3 would control the efficient translocation of virus from the inoculated leaves to the uninoculated upper leaves of radish plant.
利用由黄瓜花叶黄瓜病毒（CMV）的两个自然发生株系（CMV-D8和CMV-Y）的感染性转录本构建的假重组体，分析了黄瓜花叶黄瓜病毒（CMV）的遗传学及其对萝卜的致病性。CMV-D8能够引起植株的系统感染，而CMV-Y则不能。利用免疫组织印迹分析、斑点印迹和Northern印迹杂交技术检测了病毒在植株中的积累和系统移动情况。无论假重组体如何，病毒在萝卜接种子叶中均均匀分布，且CMV RNA积累到相似水平，这表明CMV-D8和CMV-Y在感染能力和病毒积累方面没有明显差异。然而，我们发现CMV-D8的RNA 2和RNA 3均参与决定萝卜系统感染的效率。RNA 2和RNA 3的遗传信息之间的协同相互作用将控制病毒从接种叶片向萝卜植株未接种上部叶片的有效转运。
