Adhikari Prakash B, Liu Xiaoyan, Huang Chen, Mitsuda Nobutaka, Notaguchi Michitaka, Kasahara Ryushiro Dora
Biotechnology and Bioscience Research Center, Nagoya University, Nagoya 464-8601, Japan.
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plants (Basel). 2024 Mar 31;13(7):1007. doi: 10.3390/plants13071007.
is master regulator of the molecular network involved in pollen tube attraction. Until recently, it was unclear how this gene exhibits exclusively synergid cell-specific expression in ovule. Our recent study has established that a 16-bp-long element is crucial for its synergid cell-specific expression in ovule, and an 84-bp-long fragment harboring is sufficient to drive the process. In this study, we have developed a workflow to predict functional roles of potential transcription factors (TFs) putatively binding to the promoter region, taking promoter as a test subject. After sequential assessment of co-expression pattern, network analysis, and potential master regulator identification, we have proposed a multi-TF model for regulation. Our study suggests that ANL2, GT-1, and their respective homologs could be direct regulators of and indicates that TCP15, TCP16, FRS9, and HB34 are likely master regulators of the majority of the TFs involved in its regulation. Comprehensive studies in the future are expected to offer more insights into such propositions. Developed workflow can be used while designing similar regulome-related studies for any other species and genes.
是参与花粉管吸引的分子网络的主调控因子。直到最近，还不清楚该基因如何在胚珠中仅表现出助细胞特异性表达。我们最近的研究表明，一个16个碱基对长的元件对其在胚珠中的助细胞特异性表达至关重要，并且一个包含该元件的84个碱基对长的片段足以驱动这一过程。在本研究中，我们开发了一种工作流程，以假定结合启动子区域的潜在转录因子（TFs）的功能作用为研究对象，以该启动子作为测试对象。在对共表达模式、网络分析和潜在主调控因子鉴定进行顺序评估后，我们提出了一个用于该调控的多TF模型。我们的研究表明，ANL2、GT-1及其各自的同源物可能是该基因的直接调控因子，并表明TCP15、TCP16、FRS9和HB34可能是参与其调控的大多数TFs的主调控因子。预计未来的综合研究将为这些观点提供更多见解。在为任何其他物种和基因设计类似的调控组相关研究时，可以使用所开发的工作流程。
