Yao Xiaohui, Mu Yanjie, Zhang Liyuan, Chen Lei, Zou Shenshen, Chen Xiaochen, Lu Kai, Dong Hansong
National Key Laboratory of Wheat Improvement, College of Plant Protection, Shandong Agricultural University, Taian 271018, China.
Qingdao King Agroot Crop Science, Qingdao 266071, China.
Plants (Basel). 2024 Apr 3;13(7):1018. doi: 10.3390/plants13071018.
The rapid production of hydrogen peroxide (HO) is a hallmark of plants' successful recognition of pathogen infection and plays a crucial role in innate immune signaling. Aquaporins (AQPs) are membrane channels that facilitate the transport of small molecular compounds across cell membranes. In plants, AQPs from the plasma membrane intrinsic protein (PIP) family are utilized for the transport of HO, thereby regulating various biological processes. Plants contain two PIP families, PIP1s and PIP2s. However, the specific functions and relationships between these subfamilies in plant growth and immunity remain largely unknown. In this study, we explore the synergistic role of AtPIP1;4 and AtPIP2;4 in regulating plant growth and disease resistance in Arabidopsis. We found that in plant cells treated with HO, AtPIP1;4 and AtPIP2;4 act as facilitators of HO across membranes and the translocation of externally applied HO from the apoplast to the cytoplasm. Moreover, AtPIP1;4 and AtPIP2;4 collaborate to transport bacterial pathogens and flg22-induced apoplastic HO into the cytoplasm, leading to increased callose deposition and enhanced defense gene expression to strengthen immunity. These findings suggest that AtPIP1;4 and AtPIP2;4 cooperatively mediate HO transport to regulate plant growth and immunity.
过氧化氢（H₂O₂）的快速产生是植物成功识别病原体感染的一个标志，并且在先天免疫信号传导中起着关键作用。水通道蛋白（AQP）是促进小分子化合物跨细胞膜运输的膜通道。在植物中，质膜内在蛋白（PIP）家族的水通道蛋白被用于运输H₂O₂，从而调节各种生物学过程。植物含有两个PIP家族，即PIP1s和PIP2s。然而，这些亚家族在植物生长和免疫中的具体功能及相互关系仍 largely未知。在本研究中，我们探究了拟南芥中AtPIP1;4和AtPIP2;4在调节植物生长和抗病性方面的协同作用。我们发现，在用H₂O₂处理的植物细胞中，AtPIP1;4和AtPIP2;4充当H₂O₂跨膜运输的促进因子，并使外源施加的H₂O₂从质外体转运到细胞质中。此外，AtPIP1;4和AtPIP2;4协同作用，将细菌病原体和flg22诱导的质外体H₂O₂转运到细胞质中，导致胼胝质沉积增加和防御基因表达增强，从而强化免疫。这些发现表明，AtPIP1;4和AtPIP2;4协同介导H₂O₂运输以调节植物生长和免疫。
