College of Forestry, Shandong Agricultural University, Taian 271018, China.
College of Life Sciences, Shandong Agricultural University, Taian 271018, China.
Int J Mol Sci. 2024 Mar 24;25(7):3628. doi: 10.3390/ijms25073628.
Salinity is one of the most serious threats to sustainable agriculture. The Salt Overly Sensitive (SOS) signaling pathway plays an important role in salinity tolerance in plants, and the gene plays a critical role in this pathway. Mulberry not only has important economic value but also is an important ecological tree species; however, the roles of the gene associated with salt stress have not been reported in mulberry. To gain insight into the response of mulberry to salt stress, (designated ) was cloned from mulberry ( Roxb), and sequence analysis of the amino acids of MulSOS2 showed that it shares some conserved domains with its homologs from other plant species. Our data showed that the gene was expressed at different levels in different tissues of mulberry, and its expression was induced substantially not only by NaCl but also by ABA. In addition, was exogenously expressed in Arabidopsis, and the results showed that under salt stress, transgenic plants accumulated more proline and less malondialdehyde than the wild-type plants and exhibited increased tolerance to salt stress. Moreover, the gene was transiently overexpressed in mulberry leaves and stably overexpressed in the hairy roots, and similar results were obtained for resistance to salt stress in transgenic mulberry plants. Taken together, the results of this study are helpful to further explore the function of the gene, which provides a valuable gene for the genetic breeding of salt tolerance in mulberry.
盐度是可持续农业面临的最严重威胁之一。盐过度敏感 (SOS) 信号通路在植物的耐盐性中发挥着重要作用，而该基因在该途径中起着关键作用。桑树不仅具有重要的经济价值，还是一种重要的生态树种；然而，与盐胁迫相关的基因在桑树中的作用尚未见报道。为了深入了解桑树对盐胁迫的反应，从桑树 (Roxb) 中克隆了 基因，并对 MulSOS2 氨基酸序列进行分析，表明它与其他植物物种的同源物具有一些保守结构域。我们的数据表明，该基因在桑树的不同组织中表达水平不同，其表达不仅受 NaCl 诱导，还受 ABA 诱导。此外，在拟南芥中外源表达 基因，结果表明，在盐胁迫下，转基因 植株积累的脯氨酸比野生型植株多，丙二醛含量比野生型植株少，对盐胁迫的耐受性增强。此外，该基因在桑树叶片中瞬时过表达，在毛状根中稳定过表达，在转基因桑树植株的耐盐性方面也得到了类似的结果。综上所述，本研究结果有助于进一步探索 基因的功能，为桑树耐盐遗传育种提供了有价值的基因。
