• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

顺铂耐药 MCF-7 细胞的转录组变化。

Transcriptomic Changes in Cisplatin-Resistant MCF-7 Cells.

机构信息

Laboratorio de Epigenetica, Instituto Nacional de Medicina Genomica (INMEGEN), Ciudad de Mexico 14610, Mexico.

Posgrado en Ciencias Biomédicas, Universidad Nacional Autónoma de Mexico (UNAM), Ciudad de Mexico 04510, Mexico.

出版信息

Int J Mol Sci. 2024 Mar 29;25(7):3820. doi: 10.3390/ijms25073820.

DOI:10.3390/ijms25073820
PMID:38612643
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11011657/
Abstract

Breast cancer is a leading cause of cancer-related deaths among women. Cisplatin is used for treatment, but the development of resistance in cancer cells is a significant concern. This study aimed to investigate changes in the transcriptomes of cisplatin-resistant MCF7 cells. We conducted RNA sequencing of cisplatin-resistant MCF7 cells, followed by differential expression analysis and bioinformatic investigations to identify changes in gene expression and modified signal transduction pathways. We examined the size and quantity of extracellular vesicles. A total of 724 genes exhibited differential expression, predominantly consisting of protein-coding RNAs. Notably, two long non-coding RNAs (lncRNAs), NEAT1 and MALAT, were found to be dysregulated. Bioinformatic analysis unveiled dysregulation in processes related to DNA synthesis and repair, cell cycle regulation, immune response, and cellular communication. Additionally, modifications were observed in events associated with extracellular vesicles. Conditioned media from resistant cells conferred resistance to wild-type cells in vitro. Furthermore, there was an increase in the number of vesicles in cisplatin-resistant cells. Cisplatin-resistant MCF7 cells displayed differential RNA expression, including the dysregulation of NEAT1 and MALAT long non-coding RNAs. Key processes related to DNA and extracellular vesicles were found to be altered. The increased number of extracellular vesicles in resistant cells may contribute to acquired resistance in wild-type cells.

摘要

乳腺癌是导致女性癌症相关死亡的主要原因之一。顺铂被用于治疗，但癌细胞产生耐药性是一个重大问题。本研究旨在研究顺铂耐药 MCF7 细胞的转录组变化。我们对顺铂耐药 MCF7 细胞进行了 RNA 测序，随后进行了差异表达分析和生物信息学研究，以鉴定基因表达的变化和修饰的信号转导途径。我们检查了细胞外囊泡的大小和数量。共有 724 个基因表现出差异表达，主要由蛋白质编码 RNA 组成。值得注意的是，两个长非编码 RNA（lncRNA），NEAT1 和 MALAT，被发现失调。生物信息学分析揭示了与 DNA 合成和修复、细胞周期调控、免疫反应和细胞通讯相关过程的失调。此外，还观察到与细胞外囊泡相关事件的修饰。耐药细胞的条件培养基在体外赋予野生型细胞耐药性。此外，耐药细胞中的囊泡数量增加。顺铂耐药 MCF7 细胞显示出差异的 RNA 表达，包括 NEAT1 和 MALAT 长非编码 RNA 的失调。与 DNA 和细胞外囊泡相关的关键过程被发现发生了改变。耐药细胞中外泌体数量的增加可能导致野生型细胞获得耐药性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64bc/11011657/fd2f026eb2eb/ijms-25-03820-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64bc/11011657/b607d008e12d/ijms-25-03820-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64bc/11011657/e3780ec21ffd/ijms-25-03820-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64bc/11011657/b648e233b765/ijms-25-03820-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64bc/11011657/eb6494490fb1/ijms-25-03820-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64bc/11011657/fd2f026eb2eb/ijms-25-03820-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64bc/11011657/b607d008e12d/ijms-25-03820-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64bc/11011657/e3780ec21ffd/ijms-25-03820-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64bc/11011657/b648e233b765/ijms-25-03820-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64bc/11011657/eb6494490fb1/ijms-25-03820-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64bc/11011657/fd2f026eb2eb/ijms-25-03820-g005.jpg

相似文献

1
Transcriptomic Changes in Cisplatin-Resistant MCF-7 Cells.顺铂耐药 MCF-7 细胞的转录组变化。
Int J Mol Sci. 2024 Mar 29;25(7):3820. doi: 10.3390/ijms25073820.
2
The noncoding RNA expression profile and the effect of lncRNA AK126698 on cisplatin resistance in non-small-cell lung cancer cell.非编码 RNA 表达谱及长链非编码 RNA AK126698 对非小细胞肺癌顺铂耐药的影响。
PLoS One. 2013 May 31;8(5):e65309. doi: 10.1371/journal.pone.0065309. Print 2013.
3
FOXM1 confers acquired cisplatin resistance in breast cancer cells.FOXM1 赋予乳腺癌细胞获得性顺铂耐药性。
Mol Cancer Res. 2010 Jan;8(1):24-34. doi: 10.1158/1541-7786.MCR-09-0432. Epub 2010 Jan 12.
4
Alterations of microRNAs and their targets are associated with acquired resistance of MCF-7 breast cancer cells to cisplatin.miRNAs 及其靶标的改变与 MCF-7 乳腺癌细胞对顺铂获得性耐药有关。
Int J Cancer. 2010 Oct 15;127(8):1785-94. doi: 10.1002/ijc.25191.
5
An epigenomic approach to identifying differential overlapping and cis-acting lncRNAs in cisplatin-resistant cancer cells.一种基于表观基因组学的方法，用于鉴定顺铂耐药癌细胞中差异重叠和顺式作用的长非编码 RNA。
Epigenetics. 2018;13(3):251-263. doi: 10.1080/15592294.2018.1436364. Epub 2018 Apr 2.
6
Differential Expression Profiles of the Transcriptome in Breast Cancer Cell Lines Revealed by Next Generation Sequencing.通过新一代测序揭示乳腺癌细胞系中转录组的差异表达谱。
Cell Physiol Biochem. 2017;44(2):804-816. doi: 10.1159/000485344. Epub 2017 Nov 24.
7
In-depth characterization of breast cancer tumor-promoting cell transcriptome by RNA sequencing and microarrays.通过RNA测序和微阵列对乳腺癌肿瘤促进细胞转录组进行深入表征。
Oncotarget. 2016 Jan 5;7(1):976-94. doi: 10.18632/oncotarget.5810.
8
Differences in gene expression profiles and carcinogenesis pathways involved in cisplatin resistance of four types of cancer.四种癌症顺铂耐药中涉及的基因表达谱和致癌途径的差异。
Oncol Rep. 2013 Aug;30(2):596-614. doi: 10.3892/or.2013.2514. Epub 2013 Jun 3.
9
Transcriptome Profiling of Cisplatin Resistance in Triple-negative Breast Cancer: New Insight into the Role of PI3k/Akt Pathway.三阴性乳腺癌顺铂耐药的转录组学研究：PI3K/Akt 通路作用的新见解。
Curr Mol Med. 2023;23(6):559-568. doi: 10.2174/1566524022666220517102423.
10
Chemoresistance‑related long non‑coding RNA expression profiles in human breast cancer cells.人乳腺癌细胞中与化疗耐药相关的长非编码 RNA 表达谱。
Mol Med Rep. 2018 Jul;18(1):243-253. doi: 10.3892/mmr.2018.8942. Epub 2018 Apr 27.

引用本文的文献

1
Retinoic Acid-Induced Transglutaminase 2 Expression Reduces Sensitivity to Cisplatin in the Hormone-Positive MCF-7 Breast Cancer Cell Model.维甲酸诱导的转谷氨酰胺酶2表达降低激素阳性MCF-7乳腺癌细胞模型对顺铂的敏感性。
Int J Mol Sci. 2025 Aug 21;26(16):8101. doi: 10.3390/ijms26168101.
2
Decoding LINC00052 role in breast cancer by bioinformatic and experimental analyses.通过生物信息学和实验分析解码 LINC00052 在乳腺癌中的作用。
RNA Biol. 2024 Jan;21(1):1-11. doi: 10.1080/15476286.2024.2355393. Epub 2024 Jun 4.

本文引用的文献

1
Exercise Mitigates Endothelial Pyroptosis and Atherosclerosis by Downregulating NEAT1 Through N6-Methyladenosine Modifications.运动通过下调 NEAT1 的 N6-甲基腺苷修饰来减轻内皮细胞焦亡和动脉粥样硬化。
Arterioscler Thromb Vasc Biol. 2023 Jun;43(6):910-926. doi: 10.1161/ATVBAHA.123.319251. Epub 2023 Apr 20.
2
Inhibition of lncRNA NEAT1 sensitizes medulloblastoma cells to cisplatin through modulating the miR-23a-3p-glutaminase (GLS) axis.lncRNA NEAT1 的抑制通过调节 miR-23a-3p-谷氨酰胺酶 (GLS) 轴使髓母细胞瘤细胞对顺铂敏感。
Bioengineered. 2022 Mar;13(3):7670-7682. doi: 10.1080/21655979.2021.2008695.
3
An SETD1A/Wnt/β-catenin feedback loop promotes NSCLC development.
一个 SETD1A/Wnt/β-连环蛋白反馈回路促进 NSCLC 的发展。
J Exp Clin Cancer Res. 2021 Oct 13;40(1):318. doi: 10.1186/s13046-021-02119-x.
4
Long non-coding RNA NEAT1 transported by extracellular vesicles contributes to breast cancer development by sponging microRNA-141-3p and regulating KLF12.细胞外囊泡转运的长链非编码RNA NEAT1通过吸附微小RNA-141-3p并调节KLF12促进乳腺癌发展。
Cell Biosci. 2021 Apr 5;11(1):68. doi: 10.1186/s13578-021-00556-x.
5
Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries.《全球癌症统计数据 2020：全球 185 个国家和地区 36 种癌症的发病率和死亡率估计》。
CA Cancer J Clin. 2021 May;71(3):209-249. doi: 10.3322/caac.21660. Epub 2021 Feb 4.
6
The Great Escape: The Power of Cancer Stem Cells to Evade Programmed Cell Death.《大逃脱：癌症干细胞逃避程序性细胞死亡的能力》
Cancers (Basel). 2021 Jan 17;13(2):328. doi: 10.3390/cancers13020328.
7
KEGG: integrating viruses and cellular organisms.KEGG：整合病毒和细胞生物。
Nucleic Acids Res. 2021 Jan 8;49(D1):D545-D551. doi: 10.1093/nar/gkaa970.
8
5th ESO-ESMO international consensus guidelines for advanced breast cancer (ABC 5).第五届欧洲中学教育阶段（ESO）-欧洲肿瘤内科学会（ESMO）晚期乳腺癌国际共识指南（ABC 5）
Ann Oncol. 2020 Dec;31(12):1623-1649. doi: 10.1016/j.annonc.2020.09.010. Epub 2020 Sep 23.
9
NEAT1 Knockdown Suppresses the Cisplatin Resistance in Ovarian Cancer by Regulating miR-770-5p/PARP1 Axis.NEAT1基因敲低通过调控miR-770-5p/PARP1轴抑制卵巢癌顺铂耐药性。
Cancer Manag Res. 2020 Aug 14;12:7277-7289. doi: 10.2147/CMAR.S257311. eCollection 2020.
10
LncRNA MALAT1 Regulates the Cell Proliferation and Cisplatin Resistance in Gastric Cancer via PI3K/AKT Pathway.长链非编码RNA MALAT1通过PI3K/AKT信号通路调控胃癌细胞增殖和顺铂耐药性。
Cancer Manag Res. 2020 Mar 13;12:1929-1939. doi: 10.2147/CMAR.S243796. eCollection 2020.