Group of Antioxidants and Free Radicals in Biotechnology, Food and Agriculture, Estación Experimental Zaidín (EEZ), Spanish National Research Council (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
Instrumental Technical Services of the Estación Experimental Zaidín (EEZ), Spanish National Research Council (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
Int J Mol Sci. 2024 Apr 2;25(7):3962. doi: 10.3390/ijms25073962.
The aim of the present study was to determine the ACE inhibitory activity of aqueous extracts of olive pomace and to understand whether they represent a good source of bioactive LMW peptides for nutritional and pharmacological applications. We produced a water extract from olive pomace (var. Picual) and obtained its low molecular weight (LMW) fraction (<3 kDa). The calculated yield of extraction was 100.2 ± 7.9 mg of LMW peptides per 100 g of olive pomace. The olive pomace LMW fraction possessed strong ACE inhibitory activity (IC = 3.57 ± 0.22 µg prot/mL). The LMW fraction (<3 kDa) was analysed by nanoscale liquid chromatography-Orbitrap coupled with tandem mass spectrometry and de novo sequencing. Thirty new peptides, containing between 7-17 amino acids and molecular masses ranging 778-1354 Da, were identified by the Peaks database algorithm using the available (cv. Farga) genome database. Ten new peptides were also identified by Peaks de novo sequencing. The protein sources of twelve peptides detected in the database by Peaks DB were identified by BLAST search. The ACE inhibitory activity of the identified peptides was predicted by BIOPEP software. We conclude that olive pomace possesses ACE inhibitory activity and contains low molecular weight peptides with (predicted) biological activity. Olive pomace may represent a good source of peptides for nutritional and pharmaceutical applications. In our study, it has been shown that olive pomace possesses ACE inhibitory activity and contains low molecular weight peptides with (predicted) biological activity. Olive pomace may represent a good source of peptides for nutritional and pharmaceutical applications. More research is needed in order to identify the in vivo effects of olive pomace bioactive peptides.
本研究旨在测定橄榄渣水提物的 ACE 抑制活性，并探讨其是否代表具有营养和药理应用价值的生物活性低分子量肽的良好来源。我们从橄榄渣（品种 Picual）中制备了水提取物，并获得了其低分子量（LMW）部分（<3 kDa）。计算的提取产率为每 100 克橄榄渣可获得 100.2 ± 7.9 毫克的 LMW 肽。橄榄渣 LMW 部分具有很强的 ACE 抑制活性（IC = 3.57 ± 0.22 µg prot/mL）。LMW 部分（<3 kDa）通过纳米液相色谱-Orbitrap 与串联质谱联用和从头测序进行分析。Peaks 数据库算法使用可用的（cv. Farga）基因组数据库，共鉴定出 30 种新肽，其含有 7-17 个氨基酸，分子量范围为 778-1354 Da。Peaks 从头测序还鉴定出 10 种新肽。Peaks DB 数据库中检测到的 12 种肽的蛋白质来源通过 BLAST 搜索进行鉴定。BIOPEP 软件预测了鉴定出的肽的 ACE 抑制活性。我们得出结论，橄榄渣具有 ACE 抑制活性，并且含有具有（预测）生物活性的低分子量肽。橄榄渣可能是营养和药物应用的低分子量肽的良好来源。在我们的研究中，已经表明橄榄渣具有 ACE 抑制活性，并且含有具有（预测）生物活性的低分子量肽。橄榄渣可能是营养和药物应用的低分子量肽的良好来源。需要进一步研究以确定橄榄渣生物活性肽的体内作用。
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